ArticleJournal of inorganic biochemistry2011
Pb-207 NMR spectroscopy reveals that Pb(II) coordinates with glutathione (GSH) and tris cysteine zinc finger proteins in a PbS3 coordination environment.
Article in Journal of inorganic biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The past, present, and future of artificial zinc finger proteins: design strategies and chemical and biological applications.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Pooled it
- Metal-Coordination Specificity and Structural Dynamics of C. elegans Metallothionein I: Insights From 3D Modeling and MD Simulations.Proteins · 2026Article
- Pb(II) coordination to the nonclassical zinc finger tristetraprolin: retained function with an altered fold.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Article
- Catalysis and Electron Transfer inChemical reviews · 2022Review
- Cadmium Exchange with Zinc in the Non-Classical Zinc Finger Protein Tristetraprolin.Inorganic chemistry · 2021Article
- Metal Binding Ability of Small Peptides Containing Cysteine Residues.ChemistryOpen · 2021Article
- Heteromeric three-stranded coiled coils designed using a Pb(II)(Cys)Nature chemistry · 2020Article
- Lead(II) Binding in Natural and Artificial Proteins.Metal ions in life sciences · 2017Article
- Similarities between N-Acetylcysteine and Glutathione in Binding to Lead(II) Ions.Chemical research in toxicology · 2015Article
- Sculpting Metal-binding Environments inIsrael journal of chemistry · 2015Article
- Protein design: toward functional metalloenzymes.Chemical reviews · 2014Review
- pH-dependent coordination of Pb2+ to metallothionein2: structures and insight into lead detoxification.Inorganic chemistry · 2014Article
- Probing the coordination environment of the human copper chaperone HAH1: characterization of Hg(II)-bridged homodimeric species in solution.Chemistry (Weinheim an der Bergstrasse, Germany) · 2013Article
- Lead(II) complex formation with glutathione.Inorganic chemistry · 2012Article
- The importance of stereochemically active lone pairs for influencing Pb(II) and As(III) protein binding.Chemistry (Weinheim an der Bergstrasse, Germany) · 2012Article
- Quantum-mechanical study of lead coordination in sulfur-rich proteins: mode and structure recognition in UV resonance Raman spectra.The journal of physical chemistry. A · 2012Article
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Abstract
207Pb NMR spectroscopy can be used to monitor the binding of Pb(II) to thiol rich biological small molecules such as glutathione and to zinc finger proteins. The UV/visible (UV/Vis) absorption band centered at 334 nM and the observed 207Pb-signal in 207Pb NMR (δ~5750 ppm) indicate that glutathione binds Pb(II) in a trigonal pyramidal geometry (PbS3) at pH 7.5 or higher with a 1:3 molar ratio of Pb(II) to GSH. While previous studies using UV/Vis and extended X-ray absorption fine structure (EXAFS) spectroscopy were interpreted to show that the zinc binding domain from HIV nucleocapsid protein (HIV-CCHC) binds Pb(II) in a single PbS3 environment, the more sensitive 207Pb NMR spectra (at pH 7.0, 1:1 molar ratio) provide compelling evidence for the presence of two PbS3 structures (δ=5790 and 5744 ppm), one of which is more stable at high temperatures. It has previously been proposed that the HIV-CCHH peptide does not fold properly to afford a PbS2N motif, because histidine does not bind to Pb(II). These predictions are confirmed by the present studies. These results demonstrate the applicability of 207Pb NMR to biomolecular structure determination in proteins with cysteine binding sites for the first time.
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