ArticleCellular and molecular bioengineering2011
Real Time FRET Based Detection of Mechanical Stress in Cytoskeletal and Extracellular Matrix Proteins.
Article in Cellular and molecular bioengineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 67 citations in OpenAlex.
- Pulsed ultrasound differentially stimulates somatosensory circuits in humans as indicated by EEG and FMRI.PloS one · 2012Trial
- Visualizing Cytoskeletal Protein Reconstruction of Vulvar Cancer with Surface-Enhanced Raman Spectroscopy and Gold Nanoparticles.ACS omega · 2026Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Longevity interventions modulate mechanotransduction and extracellular matrix homeostasis in C. elegans.Nature communications · 2024Article
- Using Biosensors to Study Organoids, Spheroids and Organs-on-a-Chip: A Mechanobiology Perspective.Biosensors · 2023Review
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Quantification of fast molecular adhesion by fluorescence footprinting.Science advances · 2021Article
- Mechanical Flexibility of DNA: A Quintessential Tool for DNA Nanotechnology.Sensors (Basel, Switzerland) · 2020Review
- Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Post-Turing tissue pattern formation: Advent of mechanochemistry.PLoS computational biology · 2018Article
- Emerging views of the nucleus as a cellular mechanosensor.Nature cell biology · 2018Review
- Regulation of Intracellular Structural Tension by Talin in the Axon Growth and Regeneration.Molecular neurobiology · 2016Article
- A toolbox to explore the mechanics of living embryonic tissues.Seminars in cell & developmental biology · 2016Review
- Mechanical transduction by ion channels: A cautionary tale.World journal of neurology · 2015Article
- A developmental biologist's "outside-the-cell" thinking.The Journal of cell biology · 2015Article
- Mechanical dynamics in live cells and fluorescence-based force/tension sensors.Biochimica et biophysica acta · 2015Review
- Lighting Up the Force: Investigating Mechanisms of Mechanotransduction Using Fluorescent Tension Probes.Molecular and cellular biology · 2015Review
- Article
- Basement Membranes in the Worm: A Dynamic Scaffolding that Instructs Cellular Behaviors and Shapes Tissues.Current topics in membranes · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A molecular force sensing cassette (stFRET) was incorporated into actinin, filamin, and spectrin in vascular endothelial cells (BAECs) and into collagen-19 in Caenorhabditis elegans. To estimate the stress sensitivity of stFRET in solution, we used DNA springs. A 60-mer loop of single stranded DNA was covalently linked to the external cysteines of the donor and acceptor. When the complementary DNA was added it formed double stranded DNA with higher persistence length, stretching the linker and substantially reducing FRET efficiency. The probe stFRET detected constitutive stress in all cytoskeletal proteins tested, and in migrating cells the stress was greater at the leading edge than the trailing edge. The stress in actinin, filamin and spectrin could be reduced by releasing focal attachments from the substrate with trypsin. Inhibitors of actin polymerization produced a modest increase in stress on the three proteins suggesting they are mechanically in parallel. Local shear stress applied to the cell with a perfusion pipette showed gradients of stress leading from the site of perfusion. Transgenic C. elegans labeled in collagen-19 produced a behaviorally and anatomically normal animal with constitutive stress in the cuticle. Stretching the worm visibly stretched the probe in collagen showing that we can trace the distribution of mean tissue stress in specific molecules. stFRET is a general purpose dynamic sensor of mechanical stress that can be expressed intracellularly and extracellularly in isolated proteins, cells, tissues, organs and animals.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.