ArticleCellular and molecular bioengineering2011
Difference in Energy Metabolism of Annulus Fibrosus and Nucleus Pulposus Cells of the Intervertebral Disc.
Article in Cellular and molecular bioengineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Mechanical loading of the intervertebral disc: from the macroscopic to the cellular level.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2014Pooled it
- Glycolytic-Cholesterol Subtypes of Severe Asthma Reveal Distinct Immune-Inflammatory and Metabolic Phenotypes.Journal of inflammation research · 2026Article
- Bioactive Therapies for Degenerative Disc Disease: Microenvironmental Foundations of Disease.World neurosurgery · 2025Review
- Integrating Bulk RNA and Single-Cell Sequencing Data Reveals Genes Related to Energy Metabolism and Efferocytosis in Lumbar Disc Herniation.Biomedicines · 2025Article
- Engineering extracellular matrix-based hydrogels for intervertebral disc regeneration.Frontiers in bioengineering and biotechnology · 2025Review
- In silico modeling the potential clinical effect of growth factor treatment on the metabolism of human nucleus pulposus cells.JOR spine · 2024Article
- Analysis of Extracellular ATP Distribution in the Intervertebral Disc.Annals of biomedical engineering · 2024Article
- Article
- Blocking toll-like receptor 4 mitigates static loading induced pro-inflammatory expression in intervertebral disc motion segments.Journal of biomechanics · 2023Article
- Dynamic loading leads to increased metabolic activity and spatial redistribution of viable cell density in nucleus pulposus tissue.JOR spine · 2023Article
- Controversies in spine research: Organ culture versus in vivo models for studies of the intervertebral disc.JOR spine · 2022Review
- Two- and three-dimensional in vitro nucleus pulposus cultures: An in silico analysis of local nutrient microenvironments.JOR spine · 2022Article
- AAV6 as an effective gene delivery vector for prolonged transgene expression in intervertebral disc cellsGenes & diseases · 2022Article
- Inhibition of aberrant Hif1α activation delays intervertebral disc degeneration in adult mice.Bone research · 2022Article
- Targeting mitochondrial dysfunction with small molecules in intervertebral disc aging and degeneration.GeroScience · 2021Review
- Single-cell RNA-seq identifies unique transcriptional landscapes of human nucleus pulposus and annulus fibrosus cells.Scientific reports · 2020Article
- Updated Understanding of the Degenerative Disc Diseases - Causes Versus Effects - Treatments, Studies and Hypothesis.Current genomics · 2020Review
- Effects of Oxygen Concentration and Culture Time on Porcine Nucleus Pulposus Cell Metabolism: AnFrontiers in bioengineering and biotechnology · 2019Article
- Article
- Fat Mass and Obesity-Associated (FTO) Gene Polymorphisms Are Associated with Risk of Intervertebral Disc Degeneration in Chinese Han Population: A Case Control Study.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Low back pain is associated with intervertebral disc degeneration. One of the main signs of degeneration is the inability to maintain extracellular matrix integrity. Extracellular matrix synthesis is closely related to production of adenosine triphosphate (i.e. energy) of the cells. The intervertebral disc is composed of two major anatomical regions: annulus fibrosus and nucleus pulposus, which are structurally and compositionally different, indicating that their cellular metabolisms may also be distinct. The objective of this study was to investigate energy metabolism of annulus fibrosus and nucleus pulposus cells with and without dynamic compression, and examine differences between the two cell types. Porcine annulus and nucleus tissues were harvested and enzymatically digested. Cells were isolated and embedded into agarose constructs. Dynamically loaded samples were subjected to a sinusoidal displacement at 2 Hz and 15% strain for 4 h. Energy metabolism of cells was analyzed by measuring adenosine triphosphate content and release, glucose consumption, and lactate/nitric oxide production. A comparison of those measurements between annulus and nucleus cells was conducted. Annulus and nucleus cells exhibited different metabolic pathways. Nucleus cells had higher adenosine triphosphate content with and without dynamic loading, while annulus cells had higher lactate production and glucose consumption. Compression increased adenosine triphosphate release from both cell types and increased energy production of annulus cells. Dynamic loading affected energy metabolism of intervertebral disc cells, with the effect being greater in annulus cells.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.