Evidence map›Paper›PMID 21625215›Full record

ArticleOncogene2011

microRNA-29 can regulate expression of the long non-coding RNA gene MEG3 in hepatocellular cancer.

C Braconi, T Kogure, N Valeri, N Huang, G Nuovo, S Costinean, M Negrini, E Miotto, C M Croce, T Patel

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncogene, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 364 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
364citing papers in PubMed, 4 pooled it
15.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

364 citing papers in PubMed, 4 syntheses or guidelines pooled it, 663 citations in OpenAlex.

  1. Pooled it
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  17. MicroRNAs as Biomarkers and Therapeutic Targets in Female Infertility.International journal of molecular sciences · 2024
    Review
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  20. Article

304 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

C BraconiCollege of Medicine, and the Ohio State University Comprehensive Cancer Center, Ohio State University, Columbus, OH, USA.
T Kogure
N Valeri
N Huang
G Nuovo
S Costinean
M Negrini
E Miotto
C M Croce
T Patel
The Ohio State University · USUniversity of Ferrara · ITJacksonville College · US

Funding

Translational Therapeutics Research Program (TT)P30CA016058 · NCI · OHIO STATE UNIVERSITY · PI Tasleem J Padamsee · 1985 to 2026
$132.3M
Mechanisms of Survival Signaling in Biliary EpitheliaR01DK069370 · NIDDK · SCOTT AND WHITE MEMORIAL HOSPITAL · PI PATEL, TUSHAR · 2005 to 2014
$2.4M
NCI NIH HHS P30 CA016058NIDDK NIH HHS R01 DK069370
6 · The paper itself

Abstract

The human genome is replete with long non-coding RNAs (lncRNA), many of which are transcribed and likely to have a functional role. Microarray analysis of >23,000 lncRNAs revealed downregulation of 712 (~3%) lncRNA in malignant hepatocytes, among which maternally expressed gene 3 (MEG3) was downregulated by 210-fold relative to expression in non-malignant hepatocytes. MEG3 expression was markedly reduced in four human hepatocellular cancer (HCC) cell lines compared with normal hepatocytes by real-time PCR. RNA in situ hybridization showed intense cytoplasmic expression of MEG3 in non-neoplastic liver with absent or very weak expression in HCC tissues. Enforced expression of MEG3 in HCC cells significantly decreased both anchorage-dependent and -independent cell growth, and induced apoptosis. MEG3 promoter hypermethylation was identified by methylation-specific PCR and MEG3 expression was increased with inhibition of methylation with either 5-Aza-2-Deoxycytidine, or siRNA to DNA Methyltransferase (DNMT) 1 and 3b in HCC cells. MiRNA-dependent regulation of MEG3 expression was studied by evaluating the involvement of miR-29, which can modulate DNMT 1 and 3. Overexpression of mir-29a increased expression of MEG3. GTL2, the murine homolog of MEG3, was reduced in liver tissues from hepatocyte-specific miR-29a/b1 knock-out mice compared with wild-type controls. These data show that methylation-dependent tissue-specific regulation of the lncRNA MEG3 by miR-29a may contribute to HCC growth and highlight the inter-relationship between two classes of non-coding RNA, miRNAs and lncRNAs, and epigenetic regulation of gene expression.

Indexed as

Gene Expression Regulation, NeoplasticCarcinoma, HepatocellularDNA MethylationEpigenesis, GeneticHumansLiver NeoplasmsMicroRNAsOrgan SpecificityProteinsRNA, Long NoncodingRNA, UntranslatedMEG3 non-coding RNA, humanMicroRNAsMIRN29a microRNA, humanProteinsRNA, Long NoncodingRNA, Untranslated

Identifiers

PMID21625215
PMCPMC4292930
OpenAlexW2061427860

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.