ArticleJournal of vascular research2011
Functional characterization of embryonic stem cell-derived endothelial cells.
Article in Journal of vascular research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Equine induced pluripotent stem cells are responsive to inflammatory cytokines before and after differentiation into musculoskeletal cell types.In vitro cellular & developmental biology. Animal · 2023Article
- Isolation and Characterization of Porcine Endocardial Endothelial Cells.Tissue engineering. Part C, Methods · 2023Article
- Dicer and PKR as Novel Regulators of Embryonic Stem Cell Fate and Antiviral Innate Immunity.Journal of immunology (Baltimore, Md. : 1950) · 2022Review
- Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.Communications biology · 2021Article
- Selection of different endothelialization modes and different seed cells for tissue-engineered vascular graft.Bioactive materials · 2021Review
- Functional characterization of iPSC-derived arterial- and venous-like endothelial cells.Scientific reports · 2019Article
- The underdeveloped innate immunity in embryonic stem cells: The molecular basis and biological perspectives from early embryogenesis.American journal of reproductive immunology (New York, N.Y. : 1989) · 2019Article
- Environmental Specification of Pluripotent Stem Cell Derived Endothelial Cells Toward Arterial and Venous Subtypes.Frontiers in bioengineering and biotechnology · 2019Review
- Multivalent biomaterial platform to control the distinct arterial venous differentiation of pluripotent stem cells.Biomaterials · 2018Article
- Characterization of embryonic stem cell-differentiated fibroblasts as mesenchymal stem cells with robust expansion capacity and attenuated innate immunity.Stem cell research & therapy · 2018Article
- Neuropilin-1 Mediated Arterial Differentiation of Murine Pluripotent Stem Cells.Stem cells and development · 2018Article
- Induced Pluripotent Stem Cell-Derived Endothelial Cells in Insulin Resistance and Metabolic Syndrome.Arteriosclerosis, thrombosis, and vascular biology · 2017Review
- Metabolic shift in density-dependent stem cell differentiation.Cell communication and signaling : CCS · 2017Article
- The Molecular Basis for the Lack of Inflammatory Responses in Mouse Embryonic Stem Cells and Their Differentiated Cells.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Development of Antiviral Innate Immunity During In Vitro Differentiation of Mouse Embryonic Stem Cells.Stem cells and development · 2016Article
- OCT4 Remodels the Phenotype and Promotes Angiogenesis of HUVECs by Changing the Gene Expression Profile.International journal of medical sciences · 2016Article
- Article
- Attenuated Innate Immunity in Embryonic Stem Cells and Its Implications in Developmental Biology and Regenerative Medicine.Stem cells (Dayton, Ohio) · 2015Review
- Antiviral responses in mouse embryonic stem cells: differential development of cellular mechanisms in type I interferon production and response.The Journal of biological chemistry · 2014Article
- Strategies for whole lung tissue engineering.IEEE transactions on bio-medical engineering · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Endothelial cells (EC) derived from embryonic stem cells (ESC) require additional functional characterization before they are used as a cell therapy in order to enhance their potential for engraftment and proliferation. We explore several physiologically relevant functions of ESC-derived EC (ESC-EC), such as its capacity to produce nitric oxide (NO), regulate permeability, activate and express surface molecules for the recruitment of leukocytes in response to inflammatory stimuli, migrate and grow new blood vessels, lay down extracellular matrix, and take up low-density lipoproteins. We also examined the ESC-EC ability to upregulate NO in response to shear stress and downregulate NO in response to pro-inflammatory TNF-α activation. Functional responses of ESC-EC were compared with those of cultured mouse aortic ECs. The ESC-EC exhibit most aspects of functional endothelium, but interesting differences remain. The ESC-EC produced less NO on a per cell basis, but the same amount of NO if quantified based on the area of endothelial tissue. They also exhibit increased angiogenic sprouting and are more resistant to inflammatory signals. We further characterized the subphenotype of our ESC-EC and observed both venous and arterial markers on individual cells with a larger percentage of the cells exhibiting a venous phenotype. These data support the hypothesis that the developmental default pathway is toward a venous EC, and that refinement of methods for differentiation towards arterial EC is required to maintain a homogeneous population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.