ArticleGenetics2011
The Rho1 GTPase acts together with a vacuolar glutathione S-conjugate transporter to protect yeast cells from oxidative stress.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- An ABCG Transporter Functions in Rab Localization and Lysosome-Related Organelle Biogenesis inGenetics · 2020Article
- Genome-Wide Studies of Rho5-Interacting Proteins That Are Involved in Oxidant-Induced Cell Death in Budding Yeast.G3 (Bethesda, Md.) · 2019Article
- Article
- Calcium channel regulator Mid1 links TORC2-mediated changes in mitochondrial respiration to autophagy.The Journal of cell biology · 2016Article
- Pleiotropic effects of the vacuolar ABC transporter MLT1 of Candida albicans on cell function and virulence.The Biochemical journal · 2016Article
- The accuracy of suicide statistics: are true suicide deaths misclassified?Social psychiatry and psychiatric epidemiology · 2016Article
- Fundamentals of protein interaction network mapping.Molecular systems biology · 2015Review
- Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies.Journal of molecular biology · 2015Review
- KDDN: an open-source Cytoscape app for constructing differential dependency networks with significant rewiring.Bioinformatics (Oxford, England) · 2015Article
- TOR complex 2-Ypk1 signaling regulates actin polarization via reactive oxygen species.Molecular biology of the cell · 2014Article
- YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes.Antioxidants & redox signaling · 2014Article
- Knowledge-fused differential dependency network models for detecting significant rewiring in biological networks.BMC systems biology · 2014Article
- TOR complex 2-Ypk1 signaling maintains sphingolipid homeostasis by sensing and regulating ROS accumulation.Cell reports · 2014Article
- The MAP kinase Slt2 is involved in vacuolar function and actin remodeling in Saccharomyces cerevisiae mutants affected by endogenous oxidative stress.Applied and environmental microbiology · 2013Article
- Mapping the functional yeast ABC transporter interactome.Nature chemical biology · 2013Article
- The yeast ATP-binding cassette (ABC) transporter Ycf1p enhances the recruitment of the soluble SNARE Vam7p to vacuoles for efficient membrane fusion.The Journal of biological chemistry · 2013Article
- Phosphoproteomic analysis of protein kinase C signaling in Saccharomyces cerevisiae reveals Slt2 mitogen-activated protein kinase (MAPK)-dependent phosphorylation of eisosome core components.Molecular & cellular proteomics : MCP · 2013Article
- Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis.Nature chemical biology · 2013Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- Class C ABC transporters andCellular logisticsArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Maintenance of redox homeostasis is critical for the survival of all aerobic organisms. In the budding yeast Saccharomyces cerevisiae, as in other eukaryotes, reactive oxygen species (ROS) are generated during metabolism and upon exposure to environmental stresses. The abnormal production of ROS triggers defense mechanisms to avoid the deleterious consequence of ROS accumulation. Here, we show that the Rho1 GTPase is necessary to confer resistance to oxidants in budding yeast. Temperature-sensitive rho1 mutants (rho1(ts)) are hypersensitive to oxidants and exhibit high accumulation of ROS even at a semipermissive temperature. Rho1 associates with Ycf1, a vacuolar glutathione S-conjugate transporter, which is important for heavy metal detoxification in yeast. Rho1 and Ycf1 exhibit a two-hybrid interaction with each other and form a bimolecular fluorescent complex on the vacuolar membrane. A fluorescent-based complementation assay suggests that the GTP-bound Rho1 associates with Ycf1 and that their interaction is enhanced upon exposure to hydrogen peroxide. The rho1(ts) mutants also exhibit hypersensitivity to cadmium, while cells carrying a deletion of YCF1 or mutations in a component of the Pkc1-MAP kinase pathway exhibit little or minor sensitivity to oxidants. We thus propose that Rho1 protects yeast cells from oxidative stress by regulating multiple downstream targets including Ycf1. Since both Rho1 and Ycf1 belong to highly conserved families of proteins, similar mechanisms may exist in other eukaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.