ArticleThe Journal of general physiology2011
Lens intracellular hydrostatic pressure is generated by the circulation of sodium and modulated by gap junction coupling.
Article in The Journal of general physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 104 citations in OpenAlex.
- CatExperimental eye research · 2026Article
- Extrinsic Modulation of the Zonular Tension Induces Calcium Influx in Lens Fiber Cells That Is Mediated by Mechanosensitive TRPV1 and TRPV4 Channels.Investigative ophthalmology & visual science · 2026Article
- The Mammalian Ocular Lens in Focus: Development, Anatomy, Physiology, Transparency, Biomechanics, and Age-related Challenges.Journal of visualized experiments : JoVE · 2026Review
- Regional Differences in Calcium Dynamics in the Intact Lens: The Role of TRPV Channels in Lens Calcium Regulation.Investigative ophthalmology & visual science · 2025Article
- Glymphatic Clearance in the Optic Nerve: A Multidomain Electro-Osmostic Model.Entropy (Basel, Switzerland) · 2025Article
- Studies on Hydrostatic Pressure Responses to Piezo1 and TRPV4 in the Mouse Lens.Investigative ophthalmology & visual science · 2025Article
- Compressed Cells Facilitate Adhesion Through Glycocalyx.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- CD24 is required for sustained transparency of the adult lens.Experimental eye research · 2025Article
- Biomechanical contributions to murine lens shape: Confinement, compaction, and residual stresses.Experimental eye research · 2025Article
- Optical Coherence Elastography Measures Mechanical Tension in the Lens and Capsule.Acta biomaterialia · 2025Article
- Metabolic Energy is Stored in a Homeostatic Trans-Membrane Water Barochemical Gradient.The Journal of membrane biology · 2025Article
- Modulation of the lens water content changes the stiffness of theFrontiers in ophthalmology · 2025Article
- Comparative analysis of rodent lens morphometrics and biomechanical properties.Frontiers in ophthalmology · 2025Article
- Minimizing Oxidative Stress in the Lens: Alternative Measures for Elevating Glutathione in the Lens to Protect against Cataract.Antioxidants (Basel, Switzerland) · 2024Review
- The Synergistic Effects of Polyol Pathway-Induced Oxidative and Osmotic Stress in the Aetiology of Diabetic Cataracts.International journal of molecular sciences · 2024Review
- Effect of hydrogen peroxide on lens transparency, intracellular pH, gap junction coupling, hydrostatic pressure and membrane water permeability.Experimental eye research · 2024Article
- Connexin 50 Influences the Physiological Optics of the In Vivo Mouse Lens.Investigative ophthalmology & visual science · 2024Article
- Regulation of water flow in the ocular lens: new roles for aquaporins.The Journal of physiology · 2024Review
- A Futile Cycle?: Tissue Homeostatic Trans-Membrane Water Co-Transport: Kinetics, Thermodynamics, Metabolic Consequences.bioRxiv : the preprint server for biology · 2024Article
- Tissue, cellular, and molecular level determinants for eye lens stiffness and elasticity.Frontiers in ophthalmology · 2024Review
18 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We recently modeled fluid flow through gap junction channels coupling the pigmented and nonpigmented layers of the ciliary body. The model suggested the channels could transport the secretion of aqueous humor, but flow would be driven by hydrostatic pressure rather than osmosis. The pressure required to drive fluid through a single layer of gap junctions might be just a few mmHg and difficult to measure. In the lens, however, there is a circulation of Na(+) that may be coupled to intracellular fluid flow. Based on this hypothesis, the fluid would cross hundreds of layers of gap junctions, and this might require a large hydrostatic gradient. Therefore, we measured hydrostatic pressure as a function of distance from the center of the lens using an intracellular microelectrode-based pressure-sensing system. In wild-type mouse lenses, intracellular pressure varied from ∼330 mmHg at the center to zero at the surface. We have several knockout/knock-in mouse models with differing levels of expression of gap junction channels coupling lens fiber cells. Intracellular hydrostatic pressure in lenses from these mouse models varied inversely with the number of channels. When the lens' circulation of Na(+) was either blocked or reduced, intracellular hydrostatic pressure in central fiber cells was either eliminated or reduced proportionally. These data are consistent with our hypotheses: fluid circulates through the lens; the intracellular leg of fluid circulation is through gap junction channels and is driven by hydrostatic pressure; and the fluid flow is generated by membrane transport of sodium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.