ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2011
Sequence capture and next generation resequencing of the MHC region highlights potential transplantation determinants in HLA identical haematopoietic stem cell transplantation.
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- HLA-A, -B, -C, -DRB1, -DQA1, and -DQB1 allele and haplotype frequencies defined by next generation sequencing in a population of East Croatia blood donors.Scientific reports · 2020Article
- Can Graft vs. Leukemia Effect Be Uncoupled From Graft vs. Host Disease? An Examination of Proportions.Frontiers in immunology · 2020Article
- Bioinformatics Strategies, Challenges, and Opportunities for Next Generation Sequencing-Based HLA Genotyping.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2019Review
- Intrahaplotypic Variants Differentiate Complex Linkage Disequilibrium within Human MHC Haplotypes.Scientific reports · 2015Article
- Complete haplotype phasing of the MHC and KIR loci with targeted HaploSeq.BMC genomics · 2015Article
- Routine performance and errors of 454 HLA exon sequencing in diagnostics.BMC bioinformatics · 2013Article
- Rapid, scalable and highly automated HLA genotyping using next-generation sequencing: a transition from research to diagnostics.BMC genomics · 2013Article
- Major histocompatibility complex genomics and human disease.Annual review of genomics and human genetics · 2013Review
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- Genome mapping on nanochannel arrays for structural variation analysis and sequence assembly.Nature biotechnology · 2012Article
- A comprehensively molecular haplotype-resolved genome of a European individual.Genome research · 2011Article
- Introduction to bioinformatics: sequencing technology.Asia Pacific allergy · 2011Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How cells coordinate the immune system activities is important for potentially life-saving organ or stem cell transplantations. Polymorphic immunoregulatory genes, many of them located in the human major histocompatibility complex, impact the process and assure the proper execution of tolerance-versus-activity mechanisms. In haematopoietic stem cell transplantation, on the basis of fully human leukocyte antigen (HLA)-matched donor-recipient pairs, adverse effects like graft versus leukaemia and graft versus host are observed and difficult to handle. So far, high-resolution HLA typing was performed with Sanger sequencing, but for methodological reasons information on additional immunocompetent major histocompatibility complex loci has not been revealed. Now, we have used microarray sequence capture and targeted enrichment combined with next generation pyrosequencing for 3.5 million base pair human major histocompatibility complex resequencing in a clinical transplant setting and describe 3025 variant single nucleotide polymorphisms, insertions and deletions among recipient and donor in a single sequencing experiment. Taken together, the presented data show that sequence capture and massively parallel pyrosequencing can be used as a new tool for risk assessment in the setting of allogeneic stem cell transplantation.
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