ArticleJournal of bacteriology2011
The riboswitch regulates a thiamine pyrophosphate ABC transporter of the oral spirochete Treponema denticola.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 28 citations in OpenAlex.
- The SLC-ome of membrane transport: from molecular discovery to physiology and clinical applications.Physiological reviews · 2026Review
- Borrelia burgdorferi serine protease HtrA is a pleiotropic regulator of stress response, motility, flagellar hemostasis, and infectivity.Communications biology · 2025Article
- Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogen Borrelia burgdorferi.Nature communications · 2023Article
- Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogenbioRxiv : the preprint server for biology · 2023Article
- Identification and Characterization of the Alternative σJournal of bacteriology · 2022Article
- Riboswitches as Drug Targets for Antibiotics.Antibiotics (Basel, Switzerland) · 2021Review
- Analysis of a flagellar filament cap mutant reveals that HtrA serine protease degrades unfolded flagellin protein in the periplasm of Borrelia burgdorferi.Molecular microbiology · 2019Article
- Construction of Boolean logic gates based on dual-vector circuits of multiple gene regulatory elements.Molecular genetics and genomics : MGG · 2019Article
- An in silico approach towards identification of novel drug targets in pathogenic species of Leptospira.PloS one · 2019Article
- In vivo expression technology and 5' end mapping of the Borrelia burgdorferi transcriptome identify novel RNAs expressed during mammalian infection.Nucleic acids research · 2017Article
- Illumina Sequencing Approach to Characterize Thiamine Metabolism Related Bacteria and the Impacts of Thiamine Supplementation on Ruminal Microbiota in Dairy Cows Fed High-Grain Diets.Frontiers in microbiology · 2017Article
- Lyme disease spirochaete Borrelia burgdorferi does not require thiamin.Nature microbiology · 2016Article
- Emerging applications of riboswitches - from antibacterial targets to molecular tools.Journal of applied genetics · 2016Review
- Spirochaete flagella hook proteins self-catalyse a lysinoalanine covalent crosslink for motility.Nature microbiology · 2016Article
- Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola.BMC oral health · 2016Article
- pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.Applied and environmental microbiology · 2016Article
- Evidence that TP_0144 of Treponema pallidum is a thiamine-binding protein.Journal of bacteriology · 2015Article
- Rho and RNase play a central role in FMN riboswitch regulation in Corynebacterium glutamicum.Nucleic acids research · 2015Article
- Intracellular Concentrations of Borrelia burgdorferi Cyclic Di-AMP Are Not Changed by Altered Expression of the CdaA Synthase.PloS one · 2015Article
- Molecular identification and functional characterization of the human colonic thiamine pyrophosphate transporter.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Thiamine pyrophosphate (TPP), a biologically active form of thiamine (vitamin B₁), is an essential cofactor in all living systems. Microorganisms either synthesize TPP via de novo biosynthesis pathways or uptake exogenous thiamine from the environment via specific transporters. The oral spirochete Treponema denticola is an important pathogen that is associated with human periodontal diseases. It lacks a de novo TPP biosynthesis pathway and needs exogenous TPP for growth, suggesting that it may obtain exogenous TPP via a thiamine transporter. In this study, we identified a gene cluster that encodes a TPP ABC transporter which consists of a TPP-binding protein (TDE0143), a transmembrane permease (TDE0144), and a cytosolic ATPase (TDE0145). Transcriptional and translational analyses showed that the genes encoding these three proteins are cotranscribed and form an operon (tbpABC(Td)) that is initiated by a σ⁷⁰-like promoter. The expression level of this operon is negatively regulated by exogenous TPP and is mediated by a TPP-sensing riboswitch (Td(thi-)(box)). Genetic and biochemical studies revealed that the TDE0143 deletion mutant (T. denticola ΔtbpA) had a decreased ability to transport exogenous TPP, and the mutant failed to grow when exogenous TPP was insufficient. These results taken together indicate that the tbpABC(Td) operon encodes an ABC transporter that is required for the uptake of exogenous TPP and that the expression of this operon is regulated by a TPP-binding riboswitch via a feedback inhibition mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.