ArticleNucleic acids research2011
Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
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- Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.Methods in enzymology · 2017Article
- Mechanisms of Theta Plasmid Replication.Microbiology spectrum · 2015Review
- The mutagenic footprint of low-fidelity Pol I ColE1 plasmid replication in E. coli reveals an extensive interplay between Pol I and Pol III.Current genetics · 2014Article
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- High-copy bacterial plasmids diffuse in the nucleoid-free space, replicate stochastically and are randomly partitioned at cell division.Nucleic acids research · 2014Article
- Random mutagenesis by error-prone pol plasmid replication in Escherichia coli.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Phage Lambda P protein: trans-activation, inhibition phenotypes and their suppression.Viruses · 2013Article
- Quantifying plasmid copy number to investigate plasmid dosage effects associated with directed protein evolution.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Genetics just got SEXY: Sequences encoding XY.BioengineeredArticle
- Plasmid-Mediated Antibiotic Resistance and Virulence in Gram-negatives: theMicrobiology spectrumArticle
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions. However, it is unclear how pol I and pol III work together during replication and repair or how extensive pol I processing of Okazaki fragments is in vivo. Here, we address these questions by analyzing pol I mutations generated through error-prone replication of ColE1 plasmids. The data were obtained by direct sequencing, allowing an accurate determination of the mutation spectrum and distribution. Pol I's mutational footprint suggests: (i) during leading-strand replication pol I is gradually replaced by pol III over at least 1.3 kb; (ii) pol I processing of Okazaki fragments is limited to ∼20 nt and (iii) the size of Okazaki fragments is short (∼250 nt). While based on ColE1 plasmid replication, our findings are likely relevant to other pol I replicative processes such as chromosomal replication and DNA repair, which differ from ColE1 replication mostly at the recruitment steps. This mutation footprinting approach should help establish the role of other prokaryotic or eukaryotic polymerases in vivo, and provides a tool to investigate how sequence topology, DNA damage, or interactions with protein partners may affect the function of individual DNA polymerases.
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