ArticleThe Journal of biological chemistry2011
Helix 69 is key for uniformity during substrate selection on the ribosome.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A family of ribosome hibernation factors widespread in Archaea.Nature communications · 2026Article
- Structures of naked mole-rat, tuco-tuco, and guinea pig ribosomes-is rRNA fragmentation linked to translational fidelity?Nucleic acids research · 2026Article
- Structures of Naked Mole-Rat, Tuco-Tuco, and Guinea Pig Ribosomes-Is rRNA Fragmentation Linked to Translational Fidelity?bioRxiv : the preprint server for biology · 2025Article
- Structural and mechanistic insights into the function of Leishmania ribosome lacking a single pseudouridine modification.Cell reports · 2024Article
- Disruption of evolutionarily correlated tRNA elements impairs accurate decoding.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Decoding on the ribosome depends on the structure of the mRNA phosphodiester backbone.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Small methyltransferase RlmH assembles a composite active site to methylate a ribosomal pseudouridine.Scientific reports · 2017Article
- Intersubunit Bridges of the Bacterial Ribosome.Journal of molecular biology · 2016Review
- Major centers of motion in the large ribosomal RNAs.Nucleic acids research · 2015Article
- Structure of the E. coli ribosome-EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM.Nature · 2015Article
- Distinct roles for release factor 1 and release factor 2 in translational quality control.The Journal of biological chemistry · 2014Article
- Distinct functional classes of ram mutations in 16S rRNA.RNA (New York, N.Y.) · 2014Article
- The kissing-loop T-shaped structure translational enhancer of Pea enation mosaic virus can bind simultaneously to ribosomes and a 5' proximal hairpin.Journal of virology · 2013Article
- Ligand- and pH-induced conformational changes of RNA domain helix 69 revealed by 2-aminopurine fluorescence.Angewandte Chemie (International ed. in English) · 2012Article
- Allosteric control of the ribosome by small-molecule antibiotics.Nature structural & molecular biology · 2012Article
- Role of pseudouridine in structural rearrangements of helix 69 during bacterial ribosome assembly.ACS chemical biology · 2012Article
- Rationalization and prediction of selective decoding of pseudouridine-modified nonsense and sense codons.RNA (New York, N.Y.) · 2012Article
- Modulation of conformational changes in helix 69 mutants by pseudouridine modifications.Biophysical chemistryArticle
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2 authors.
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Abstract
Structural studies of ribosome complexes with bound tRNAs and release factors show considerable contacts between these factors and helix 69 (H69) of 23 S rRNA. Although biochemical and genetic studies have provided some general insights into the role of H69 in tRNA and RF selection, a detailed understanding of these contributions remains elusive. Here, we present a pre- steady-state kinetic analysis establishing that two distinct regions of H69 make critical contributions to substrate selection. The loop of H69 (A1913) forms contacts necessary for the efficient accommodation of a subset of natural tRNA species, whereas the base of the stem (G1922) is specifically critical for UGA codon recognition by the class 1 release factor RF2. These data define a broad and critical role for this centrally located intersubunit helix (H69) in accurate and efficient substrate recognition by the ribosome.
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