ArticleAnalytical biochemistry2011
A continuous fluorescence displacement assay for BioA: an enzyme involved in biotin biosynthesis.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- Structure-Guided Development of a Potent BioA Inhibitor Validates Biotin Synthesis Inhibition as a Therapeutic Strategy for Tuberculosis.bioRxiv : the preprint server for biology · 2025Article
- Advances and prospects in microbial production of biotin.Microbial cell factories · 2024Review
- Quantifying the Interactions between Biomolecules: Guidelines for Assay Design and Data Analysis.ACS infectious diseases · 2019Article
- Identification ofDrug design, development and therapy · 2018Article
- Synthesis of a 3-Amino-2,3-dihydropyrid-4-one and Related Heterocyclic Analogues as Mechanism-Based Inhibitors of BioA, a Pyridoxal Phosphate-Dependent Enzyme.The Journal of organic chemistry · 2017Article
- Structure-Based Optimization of Pyridoxal 5'-Phosphate-Dependent Transaminase Enzyme (BioA) Inhibitors that Target Biotin Biosynthesis in Mycobacterium tuberculosis.Journal of medicinal chemistry · 2017Article
- Inhibition of Inflammatory and Neuropathic Pain by Targeting a Mu Opioid Receptor/Chemokine Receptor5 Heteromer (MOR-CCR5).Journal of medicinal chemistry · 2015Article
- Fragment-based exploration of binding site flexibility in Mycobacterium tuberculosis BioA.Journal of medicinal chemistry · 2015Article
- Target-based identification of whole-cell active inhibitors of biotin biosynthesis in Mycobacterium tuberculosis.Chemistry & biology · 2015Article
- Inhibition of Mycobacterium tuberculosis transaminase BioA by aryl hydrazines and hydrazides.Chembiochem : a European journal of chemical biology · 2014Article
- Design and synthesis of potential mechanism-based inhibitors of the aminotransferase BioA involved in biotin biosynthesis.The Journal of organic chemistry · 2012Article
- Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Mechanism-based inactivation by aromatization of the transaminase BioA involved in biotin biosynthesis in Mycobaterium tuberculosis.Journal of the American Chemical Society · 2011Article
- Evaluating the sensitivity of Mycobacterium tuberculosis to biotin deprivation using regulated gene expression.PLoS pathogens · 2011Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Cofactor biosynthetic pathways represent a rich source of potential antibiotic targets. The second step in biotin biosynthesis is performed by BioA, a pyridoxal 5'-phosphate (PLP)-dependent enzyme. This enzyme has been confirmed as a candidate target in Mycobacterium tuberculosis; however, the current bioassay used to measure BioA activity is cumbersome and low throughput. Here we describe the design, development, and optimization of a continuous coupled fluorescence displacement assay to measure BioA activity. In this coupled assay, BioD converts the product of the BioA-catalyzed reaction into dethiobiotin, which is subsequently detected by displacement of a fluorescently labeled dethiobiotin probe from streptavidin. The assay was further adapted to a high-throughput screening format and validated against the LOPAC(1280) library.
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