ArticleBMC microbiology2011
Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The Impacts of Essential Gcp/TsaD Protein on Cell Morphology, Virulence Expression, and Antibiotic Susceptibility inMicroorganisms · 2025Article
- New Insights into the Biological Functions of Essential TsaB/YeaZ Protein inAntibiotics (Basel, Switzerland) · 2024Article
- Protein aggregates act as a deterministic disruptor during bacterial cell size homeostasis.Cellular and molecular life sciences : CMLS · 2023Article
- Many birds with one stone: targeting the (p)ppGpp signaling pathway of bacteria to improve antimicrobial therapy.Biophysical reviews · 2021Review
- Functions of Bacterial tRNA Modifications: From Ubiquity to Diversity.Trends in microbiology · 2021Review
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- Individual- versus group-optimality in the production of secreted bacterial compounds.Evolution; international journal of organic evolution · 2019Article
- Image analysis driven single-cell analytics for systems microbiology.BMC systems biology · 2017Article
- Bet-hedging in bacteriocin producing Escherichia coli populations: the single cell perspective.Scientific reports · 2017Article
- Article
- Essentiality of threonylcarbamoyladenosine (t(6)A), a universal tRNA modification, in bacteria.Molecular microbiology · 2015Article
- Effects of (p)ppGpp on the progression of the cell cycle of Caulobacter crescentus.Journal of bacteriology · 2014Article
- Article
- Patterns of evolutionary conservation of essential genes correlate with their compensability.PLoS genetics · 2012Article
- Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside.The Journal of biological chemistry · 2012Article
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Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe essential Escherichia coli gene ygjD belongs to a universally conserved group of genes whose function has been the focus of a number of recent studies. Here, we put ygjD under control of an inducible promoter, and used time-lapse microscopy and single cell analysis to investigate the phenotypic consequences of the depletion of YgjD protein from growing cells.
resultsWe show that loss of YgjD leads to a marked decrease in cell size and termination of cell division. The transition towards smaller size occurs in a controlled manner: cell elongation and cell division remain coupled, but cell size at division decreases. We also find evidence that depletion of YgjD leads to the synthesis of the intracellular signaling molecule (p)ppGpp, inducing a cellular reaction resembling the stringent response. Concomitant deletion of the relA and spoT genes - leading to a strain that is uncapable of synthesizing (p)ppGpp - abrogates the decrease in cell size, but does not prevent termination of cell division upon YgjD depletion.
conclusionsDepletion of YgjD protein from growing cells leads to a decrease in cell size that is contingent on (p)ppGpp, and to a termination of cell division. The combination of single-cell timelapse microscopy and statistical analysis can give detailed insights into the phenotypic consequences of the loss of essential genes, and can thus serve as a new tool to study the function of essential genes.
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