Evidence map›Paper›PMID 21618086›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2011

Imaging of embryonic stem cell migration in vivo.

Andrew S Lee, Joseph C Wu

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Pluripotent stem cell derived cardiomyocytes for cardiac repair.Current treatment options in cardiovascular medicine · 2014
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Andrew S LeeDivision of Cardiology, Department of Medicine, Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
Joseph C Wu
Stanford University · US

Funding

Nanostructuring and Molecular Imaging of Engineered Cardiovascular TissuesR33HL089027 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2009 to 2011
$2.3M
Nanostructuring and Molecular Imaging of Engineered Cardiovascular TissuesR21HL089027 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2007 to 2008
$777k
Biological Insights into Dynamics of Stem Cell Differentiation and MisbehaviorR21HL091453 · NHLBI · STANFORD UNIVERSITY · PI WU, JOSEPH C. · 2008 to 2009
$719k
Howard Hughes Medical InstituteNHLBI NIH HHS R21 HL089027NHLBI NIH HHS R21 HL091453NHLBI NIH HHS R21/R33 HL089027NHLBI NIH HHS R33 HL089027
6 · The paper itself

Abstract

Conventional reporter gene technology and histological methods cannot routinely be used to track the in vivo behavior of embryonic stem (ES) cells longitudinally after cellular transplantation. Here we describe a protocol for monitoring the in vivo survival, proliferation, and migration of ES cells without necessitating animal sacrifice. Stable ES cell lines containing double fusion (DF; enhanced green fluorescent protein and firefly luciferase) or triple fusion (TF; monomeric red fluorescent protein, firefly luciferase, and herpes simplex virus thymidine kinase) reporter genes can be established within 4-6 weeks by lentiviral transduction followed by fluorescence-activated cell sorting. The cell fate and behavior of these DF or TF ES cells can subsequently be tracked noninvasively by bioluminescence and microPET imaging for a prolonged period of time.

Indexed as

Genes, ReporterStem Cell TransplantationAnimalsCell SurvivalEmbryonic Stem CellsFlow CytometryFluorescenceGreen Fluorescent ProteinsHEK293 CellsHumansLentivirusLuciferases, FireflyLuminescent MeasurementsLuminescent ProteinsMiceMice, Inbred C57BLenhanced green fluorescent proteinGreen Fluorescent ProteinsLuciferases, FireflyLuminescent ProteinsRecombinant Fusion ProteinsRed Fluorescent ProteinThymidine Kinase

Identifiers

PMID21618086
PMCPMC3657515
OpenAlexW1482025560

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.