ArticlePlant signaling & behavior2011
The activation of the Arabidopsis P-ATPase 1 by the brassinosteroid receptor BRI1 is independent of threonine 948 phosphorylation.
Article in Plant signaling & behavior, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 23 citations in OpenAlex.
- Preliminary Treatment by Exogenous 24-Epibrassinolide Influences Burning-Induced Electrical Signals and Following Photosynthetic Responses in Pea (Plants (Basel, Switzerland) · 2024Article
- OneFlowTraX: a user-friendly software for super-resolution analysis of single-molecule dynamics and nanoscale organization.Frontiers in plant science · 2024Article
- Three-Fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells.Plants (Basel, Switzerland) · 2022Article
- Article
- Integrating Phosphoproteomics and Bioinformatics to Study Brassinosteroid-Regulated Phosphorylation Dynamics in Arabidopsis.BMC genomics · 2015Article
- Expression of a translationally fused TAP-tagged plasma membrane proton pump in Arabidopsis thaliana.Biochemistry · 2014Article
- The brassinosteroid insensitive1-like3 signalosome complex regulates Arabidopsis root development.The Plant cell · 2013Article
- Latest news on Arabidopsis brassinosteroid perception and signaling.Frontiers in plant science · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plasma membrane-spanning receptor brassinosteroid insenstive 1 (BRI1) rapidly induces plant cell wall expansion in response to brassinosteroids such as brassinolide (BL). Wall expansion is accompanied by a rapid hyperpolarisation of the plasma membrane which is recordable by measuring the fluorescence lifetime (FLT) of the green fluorescent protein (GFP) fused to BRI1. For the BL induction of hyperpolarisation and wall expansion, the activation of the plasma membrane P-type H+-ATPase is necessary. Furthermore, the activation of the P-ATPase requires BRI1 kinase activity and appears to be mediated by a BL-modulated association of BRI1 with the proton pump. Here, we show that BRI1 also associates with a mutant version of the Arabidopsis P-ATPase 1 (AHA1) characterized by an exchange of a well-known regulatory threonine for a non-phosphorylatable residue in the auto-inhibitory C-terminal domain. Even more important, BRI1 is still able to activate this AHA1 mutant in response to BL. This suggests a novel mechanism for the enzymatic activation of the P-ATPase by BRI1 in the plasma membrane. Furthermore, we demonstrate that the FLT of BRI1-GFP can be used as a non-invasive probe to analyse long-distance BL signaling in Arabidopsis seedlings.
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