ArticleDiabetes2011
Genetic defect in phospholipase Cδ1 protects mice from obesity by regulating thermogenesis and adipogenesis.
Article in Diabetes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Trans-ethnic meta-analysis of rare variants in sequencing association studies.Biostatistics (Oxford, England) · 2021Pooled it
- Antisense oligonucleotide-mediated knockdown of Mpzl3 attenuates the negative metabolic effects of diet-induced obesity in mice.Physiological reports · 2021Article
- PLCD1: A Potential Therapeutic Target in the Treatment of Esophageal Squamous Cell Carcinoma?Digestive diseases and sciences · 2021Article
- Spinophilin-deficient mice are protected from diet-induced obesity and insulin resistance.American journal of physiology. Endocrinology and metabolism · 2020Article
- Review
- Effects of exercise on brown and beige adipocytes.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019Review
- Foxn1 in Skin Development, Homeostasis and Wound Healing.International journal of molecular sciences · 2018Review
- Warming the mouse to model human diseases.Nature reviews. Endocrinology · 2017Review
- High-intensity interval training and calorie restriction promote remodeling of glucose and lipid metabolism in diet-induced obesity.American journal of physiology. Endocrinology and metabolism · 2017Article
- Phospholipase C-related Catalytically Inactive Protein Is a New Modulator of Thermogenesis Promoted by β-Adrenergic Receptors in Brown Adipocytes.The Journal of biological chemistry · 2016Article
- Association between DNA Methylation in Whole Blood and Measures of Glucose Metabolism: KORA F4 Study.PloS one · 2016Article
- Article
- Review
- Integration of body temperature into the analysis of energy expenditure in the mouse.Molecular metabolism · 2015Article
- Simultaneous loss of phospholipase Cδ1 and phospholipase Cδ3 causes cardiomyocyte apoptosis and cardiomyopathy.Cell death & disease · 2014Article
- G protein-coupled receptors and adipogenesis: a focus on adenosine receptors.Journal of cellular physiology · 2014Review
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- Mechanisms of obesity-induced inflammation and insulin resistance: insights into the emerging role of nutritional strategies.Frontiers in endocrinology · 2013Article
- Article
- Alopecia in a viable phospholipase C delta 1 and phospholipase C delta 3 double mutant.PloS one · 2012Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveRegulation of obesity development is an important issue to prevent metabolic syndromes. Gene-disrupted mice of phospholipase Cδ1 (PLCδ1), a key enzyme of phosphoinositide turnover, seemed to show leanness. Here we examined whether and how PLCδ1 is involved in obesity. RESEARCH DESIGN AND
methodsWeight gain, insulin sensitivity, and metabolic rate in PLCδ1(-/-) mice were compared with PLCδ1(+/-) littermate mice on a high-fat diet. Thermogenic and adipogenetic potentials of PLCδ1(-/-) immortalized brown adipocytes and adipogenesis of PLCδ1-knockdown (KD) 3T3L1 cells, or PLCδ1(-/-) white adipose tissue (WAT) stromal-vascular fraction (SVF) cells, were also investigated.
resultsPLCδ1(-/-) mice showed marked decreases in weight gain and mass of epididymal WAT and preserved insulin sensitivity compared with PLCδ1(+/-) mice on a high-fat diet. In addition, PLCδ1(-/-) mice have a higher metabolic rate such as higher oxygen consumption and heat production. When control immortalized brown adipocytes were treated with thermogenic inducers, expression of PLCδ1 was decreased and thermogenic gene uncoupling protein 1 (UCP1) was upregulated to a greater extent in PLCδ1(-/-) immortalized brown adipocytes. In contrast, ectopic expression of PLCδ1 in PLCδ1(-/-) brown adipocytes induced a decrease in UCP expression, indicating that PLCδ1 negatively regulates thermogenesis. Importantly, accumulation of lipid droplets was severely decreased when PLCδ1-KD 3T3L1 cells, or PLCδ1(-/-) WAT SVF cells, were differentiated, whereas differentiation of PLCδ1(-/-) brown preadipocytes was promoted.
conclusionsPLCδ1 has essential roles in thermogenesis and adipogenesis and thereby contributes to the development of obesity.
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