ArticlePlant physiology2011
Quantification of the brassinosteroid insensitive1 receptor in planta.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- SIZ1 SUMOylates and stabilizes WRI1 to safeguard seed filling and fatty acid biosynthesis under high-temperature stress.The Plant cell · 2025Article
- Stem cell quiescence and dormancy in plant meristems.Journal of experimental botany · 2024Review
- Article
- Review
- Impact of explicit area scaling on kinetic models involving multiple compartments.BMC bioinformatics · 2021Article
- Hormonal Regulation of Stem Cell Proliferation at theFrontiers in plant science · 2021Review
- TTL Proteins Scaffold Brassinosteroid Signaling Components at the Plasma Membrane to Optimize Signal Transduction in Arabidopsis.The Plant cell · 2019Article
- Paracrine brassinosteroid signaling at the stem cell niche controls cellular regeneration.Journal of cell science · 2018Article
- Effect of probe diffusion on the SOFI imaging accuracy.Scientific reports · 2017Article
- Article
- Differential expression of the brassinosteroid receptor-encoding BRI1 gene in Arabidopsis.Planta · 2014Article
- A mathematical model for the coreceptors SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE3 in BRASSINOSTEROID INSENSITIVE1-mediated signaling.Plant physiology · 2013Article
- The brassinosteroid insensitive1-like3 signalosome complex regulates Arabidopsis root development.The Plant cell · 2013Article
- Visualization of BRI1 and BAK1(SERK3) membrane receptor heterooligomers during brassinosteroid signaling.Plant physiology · 2013Article
- The brassinosteroid signaling pathway-new key players and interconnections with other signaling networks crucial for plant development and stress tolerance.International journal of molecular sciences · 2013Review
- A mathematical model for BRASSINOSTEROID INSENSITIVE1-mediated signaling in root growth and hypocotyl elongation.Plant physiology · 2012Article
- Brassinosteroids inhibit pathogen-associated molecular pattern-triggered immune signaling independent of the receptor kinase BAK1.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- A model for the biosynthesis and transport of plasma membrane-associated signaling receptors to the cell surface.Frontiers in plant science · 2012Article
- Latest news on Arabidopsis brassinosteroid perception and signaling.Frontiers in plant science · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, green fluorescent protein (GFP) is routinely used to determine the subcellular location of fusion proteins. Here, we show that confocal imaging can be employed to approximate the number of GFP-labeled protein molecules present in living Arabidopsis (Arabidopsis thaliana) root cells. The technique involves calibration with soluble GFP to provide a usable protein concentration range within the confocal volume of the microscope. As a proof of principle, we quantified the Brassinosteroid Insensitive1 (BRI1) receptor fused to GFP, under control of its own promoter. The number of BRI1-GFP molecules per root epidermal cell ranges from 22,000 in the meristem and 130,000 in the elongation zone to 80,000 in the maturation zone, indicating that up to 6-fold differences in BRI1 receptor content exist. In contrast, when taking into account differences in cell size, BRI1-GFP receptor density in the plasma membrane is kept constant at 12 receptors μm⁻² in all cells throughout the meristem and elongation zone. Only the quiescent center and columella cells deviate from this pattern and have 5 to 6 receptors μm⁻². Remarkably, root cell sensitivity toward brassinosteroids appears to coincide with uniform meristem receptor density.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.