Evidence map›Paper›PMID 21615153›Full record

ArticleMolecular pharmaceutics2011

Negatively charged liposomes show potent adjuvant activity when simply admixed with protein antigens.

Nijaporn Yanasarn, Brian R Sloat, Zhengrong Cui

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular pharmaceutics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 48 citations in OpenAlex.

  1. Review
  2. Review
  3. The combination of MnODrug delivery · 2022
    Article
  4. Liposomes as Adjuvants and Vaccine Delivery Systems.Biochemistry (Moscow) Supplement. Series A, Membrane and cell biology · 2022
    Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Liposomes used as a vaccine adjuvant-delivery system: From basics to clinical immunization.Journal of controlled release : official journal of the Controlled Release Society · 2019
    Review
  11. Review
  12. Review
  13. Article
  14. Review
  15. Adjuvant formulation structure and composition are critical for the development of an effective vaccine against tuberculosis.Journal of controlled release : official journal of the Controlled Release Society · 2013
    Article
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Nijaporn YanasarnPharmaceutics Division, College of Pharmacy, The University of Texas at Austin, Austin, Texas 78712, USA.
Brian R Sloat
Zhengrong Cui
The University of Texas at Austin · USPediatrics and Genetics · US

Funding

Topical DNA immunization by modifying the hair follicle cycleR01AI078304 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI CUI, ZHENGRONG · 2008 to 2011
$1.4M
A tri-antigen nasal anthrax vaccine against toxins and vegetative bacilli R21AI070538 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI CUI, ZHENGRONG · 2007 to 2008
$379k
NIAID NIH HHS AI070538NIAID NIH HHS AI078304NIAID NIH HHS R01 AI078304NIAID NIH HHS R21 AI070538
6 · The paper itself

Abstract

Liposomes have been investigated extensively as a vaccine delivery system. Herein the adjuvant activities of liposomes with different net surface charges (neutral, positive, or negative) were evaluated when admixed with protein antigens, ovalbumin (OVA, pI = 4.7), Bacillus anthracis protective antigen protein (PA, pI = 5.6), or cationized OVA (cOVA). Mice immunized subcutaneously with OVA admixed with different liposomes generated different antibody responses. Interestingly, OVA admixed with net negatively charged liposomes prepared with DOPA was as immunogenic as OVA admixed with positively charged liposomes prepared with DOTAP. Immunization of mice with the anthrax PA protein admixed with the net negatively charged DOPA liposomes also induced a strong and functional anti-PA antibody response. When the cationized OVA was used as a model antigen, liposomes with net neutral, negative, or positive charges showed comparable adjuvant activities. Immunization of mice with the OVA admixed with DOPA liposomes also induced OVA-specific CD8(+) cytotoxic T lymphocyte responses and significantly delayed the growth of OVA-expressing B16-OVA tumors in mice. However, not all net negatively charged liposomes showed a strong adjuvant activity. The adjuvant activity of the negatively charged liposomes may be related to the liposome's ability (i) to upregulate the expression of molecules related to the activation and maturation of antigen-presenting cells and (ii) to slightly facilitate the uptake of the antigens by antigen-presenting cells. Simply admixing certain negatively charged liposomes with certain protein antigens of interest may represent a novel platform for vaccine development.

Indexed as

Adjuvants, ImmunologicAnimalsAntibody FormationAntigensAntigens, BacterialBacterial ToxinsFemaleLiposomesMiceMice, Inbred BALB CMice, Inbred C57BLOvalbuminProteinsAdjuvants, Immunologicanthrax toxinAntigensAntigens, BacterialBacterial ToxinsLiposomesOvalbuminProteins

Identifiers

PMID21615153
PMCPMC3148289
OpenAlexW2079721126

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.