ArticleBiochemistry2011
Quantification of cysteinyl S-nitrosylation by fluorescence in unbiased proteomic studies.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Sildenafil increases muscle protein synthesis and reduces muscle fatigue.Clinical and translational science · 2013Trial
- S-Nitrosylation of p53 in Melanoma Cells Under Nitrosative Stress.International journal of molecular sciences · 2025Article
- Hsp70 in Redox Homeostasis.Cells · 2022Review
- Cancer and Associated Therapies Impact the Skeletal Muscle Proteome.Frontiers in physiology · 2022Article
- iNOS Associates With Poor Survival in Melanoma: A Role for Nitric Oxide in the PI3K-AKT Pathway Stimulation and PTEN S-Nitrosylation.Frontiers in oncology · 2021Article
- Article
- Potential Utility of Protein Targets of Cysteine-S-Nitrosylation in Identifying Clinical Disease Status in Human Chagas Disease.Frontiers in microbiology · 2018Article
- Postsynaptic Proteome of Non-Demented Individuals with Alzheimer's Disease Neuropathology.Journal of Alzheimer's disease : JAD · 2018Article
- S-Nitrosylated fetal hemoglobin in neonatal human blood.Biochemical and biophysical research communications · 2016Article
- Glutathionylation of the Bacterial Hsp70 Chaperone DnaK Provides a Link between Oxidative Stress and the Heat Shock Response.The Journal of biological chemistry · 2016Article
- Changes in Proteome Profile of Peripheral Blood Mononuclear Cells in Chronic Chagas Disease.PLoS neglected tropical diseases · 2016Article
- S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure.International journal of proteomics · 2016Article
- Strategies for profiling native S-nitrosylation.Biopolymers · 2014Review
- NitroDIGE analysis reveals inhibition of protein S-nitrosylation by epigallocatechin gallates in lipopolysaccharide-stimulated microglial cells.Journal of neuroinflammation · 2014Article
- Molecular pathways: inflammation-associated nitric-oxide production as a cancer-supporting redox mechanism and a potential therapeutic target.Clinical cancer research : an official journal of the American Association for Cancer Research · 2013Review
- The impact of biosampling procedures on molecular data interpretation.Molecular & cellular proteomics : MCP · 2013Review
- Ventilatory responses during and following exposure to a hypoxic challenge in conscious mice deficient or null in S-nitrosoglutathione reductase.Respiratory physiology & neurobiology · 2013Article
- Nitric oxide-based protein modification: formation and site-specificity of protein S-nitrosylation.Frontiers in plant science · 2013Article
- Host S-nitrosylation inhibits clostridial small molecule-activated glucosylating toxins.Nature medicine · 2011Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Cysteinyl S-nitrosylation has emerged as an important post-translational modification affecting protein function in health and disease. Great emphasis has been placed on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidative stimuli. However, current strategies have been hampered by sample loss and altered protein electrophoretic mobility. Here, we describe a novel quantitative approach that uses accurate, sensitive fluorescence modification of cysteine S-nitrosylation that leaves electrophoretic mobility unaffected (SNOFlo) and introduce unique concepts for measuring changes in S-nitrosylation status relative to protein abundance. Its efficacy in defining the functional S-nitrosoproteome is demonstrated in two diverse biological applications: an in vivo rat hypoxia-ischemia/reperfusion model and antimicrobial S-nitrosoglutathione-driven transnitrosylation of an enteric microbial pathogen. The suitability of this approach for investigating endogenous S-nitrosylation is further demonstrated using Ingenuity Pathways analysis that identified nervous system and cellular development networks as the top two networks. Functional analysis of differentially S-nitrosylated proteins indicated their involvement in apoptosis, branching morphogenesis of axons, cortical neurons, and sympathetic neurites, neurogenesis, and calcium signaling. Major abundance changes were also observed for fibrillar proteins known to be stress-responsive in neurons and glia. Thus, both examples demonstrate the technique's power in confirming the widespread involvement of S-nitrosylation in hypoxia-ischemia/reperfusion injury and in antimicrobial host responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.