ReviewFolia microbiologica2011
Gene replacement techniques for Escherichia coli genome modification.
Review in Folia microbiologica, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- CnRed: Efficient, Marker-free Genome Engineering ofACS synthetic biology · 2025Article
- Modular reconstruction and optimization of the trans-4-hydroxy-L-proline synthesis pathway in Escherichia coli.Microbial cell factories · 2022Article
- Type I fimbriae subunitFrontiers in bioengineering and biotechnology · 2022Article
- Optimized gene expression from bacterial chromosome by high-throughput integration and screening.Science advances · 2021Article
- Extension of Genetic Marker List Using Unnatural Amino Acid System: An Efficient Genomic Modification Strategy inFrontiers in bioengineering and biotechnology · 2020Article
- Efficient Biofilm-Based Fermentation Strategies for L-Threonine Production byFrontiers in microbiology · 2019Article
- Oligo- and dsDNA-mediated genome editing using a tetA dual selection system in Escherichia coli.PloS one · 2017Article
- Mechanisms of gene targeting in higher eukaryotes.Cellular and molecular life sciences : CMLS · 2016Review
- L-Tryptophan Production in Escherichia coli Improved by Weakening the Pta-AckA Pathway.PloS one · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The subject of this review covers modern experimental procedures for chromosomal gene replacement in Escherichia coli and related bacteria, which enable the specific substitution of targeted genome sequences with copies of those carrying defined mutations. Two principal methods for gene replacement were established. The first "in-out" method is based on integration of plasmid into bacterial chromosome and subsequent resolving of the cointegrate. The "linear fragment" method (recombineering) is based on homologous recombination mediated by short homology arms at the ends of linear DNA molecule. Many new protocols and improvements in targeted gene replacement were introduced during the last 10 years. These methods are well suited for high-throughput functional gene studies and for many biotechnological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.