ArticleMolecular biology of the cell2011
Axonal filopodial asymmetry induced by synaptic target.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Distinct postsynaptic morphogenetic strategies acrossbioRxiv : the preprint server for biology · 2026Article
- Engineering skeletal muscle tissues with advanced maturity improves synapse formation with human induced pluripotent stem cell-derived motor neurons.APL bioengineering · 2021Article
- A novel fibroblast growth factor receptor family member promotes neuronal outgrowth and synaptic plasticity in aplysia.Amino acids · 2014Article
- Heparanase 2, mutated in urofacial syndrome, mediates peripheral neural development in Xenopus.Human molecular genetics · 2014Article
- Simultaneous pre- and post-synaptic electrophysiological recording from Xenopus nerve-muscle co-cultures.Journal of visualized experiments : JoVE · 2013Article
- Nerve growth factor-induced formation of axonal filopodia and collateral branches involves the intra-axonal synthesis of regulators of the actin-nucleating Arp2/3 complex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Regulation of axonal growth and neuromuscular junction formation by neuronal phosphatase and tensin homologue signaling.Molecular biology of the cell · 2012Article
- The function of p120 catenin in filopodial growth and synaptic vesicle clustering in neurons.Molecular biology of the cell · 2012Article
- Epitope-tagged dopamine transporter knock-in mice reveal rapid endocytic trafficking and filopodia targeting of the transporter in dopaminergic axons.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Reciprocal regulation of axonal Filopodia and outgrowth during neuromuscular junction development.PloS one · 2012Article
- Analysis of the fibroblast growth factor system reveals alterations in a mouse model of spinal muscular atrophy.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During vertebrate neuromuscular junction (NMJ) assembly, motor axons and their muscle targets exchange short-range signals that regulate the subsequent steps of presynaptic and postsynaptic specialization. We report here that this interaction is in part mediated by axonal filopodia extended preferentially by cultured Xenopus spinal neurons toward their muscle targets. Immunoblotting and labeling experiments showed that basic fibroblast growth factor (bFGF) was expressed by muscle and associated with the cell surface, and treatment of cultured spinal neurons with recombinant bFGF nearly doubled the normal density of filopodia in neurites. This effect of bFGF was abolished by SU5402, a selective inhibitor of FGF-receptor 1 (FGFR1), and forced expression of wild-type or dominant-negative FGFR1 in neurons enhanced or suppressed the assembly of filopodia, respectively. Significantly, in nerve-muscle cocultures, knocking down bFGF in muscle decreased both the asymmetric extension of filopodia by axons toward muscle and the assembly of NMJs. In addition, neurons expressing dominant-negative FGFR1 less effectively triggered the aggregation of muscle acetylcholine receptors at innervation sites than did control neurons. These results suggest that bFGF activation of neuronal FGFR1 generates filopodial processes in neurons that promote nerve-muscle interaction and facilitate NMJ establishment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.