ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
The CD59 family member Leaky/Coiled is required for the establishment of the blood-brain barrier in Drosophila.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Invertebrate glial barriers as a model for understanding blood-brain barrier evolution.Fluids and barriers of the CNS · 2025Review
- Endocytosis at the Crossroad of Polarity and Signaling Regulation: Learning fromInternational journal of molecular sciences · 2022Review
- Expanding the Junction: New Insights into Non-Occluding Roles for Septate Junction Proteins during Development.Journal of developmental biology · 2021Review
- Dynamic analysis of the mesenchymal-epithelial transition of blood-brain barrier forming glia inBiology open · 2017Article
- Boudin trafficking reveals the dynamic internalisation of specific septate junction components in Drosophila.PloS one · 2017Article
- Multispecies Analysis of Expression Pattern Diversification in the Recently Expanded Insect Ly6 Gene Family.Molecular biology and evolution · 2015Article
- Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.Development (Cambridge, England) · 2014Article
- Barrier mechanisms in the Drosophila blood-brain barrier.Frontiers in neuroscience · 2014Review
- The Drosophila blood-brain barrier: development and function of a glial endothelium.Frontiers in neuroscience · 2014Review
- The Drosophila surface glia transcriptome: evolutionary conserved blood-brain barrier processes.Frontiers in neuroscience · 2014Article
- The urokinase receptor homolog Haldisin is a novel differentiation marker of stratum granulosum in squamous epithelia.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
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5 authors.
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Abstract
The blood-brain barrier of Drosophila is established by the subperineurial glial cells that encase the CNS and PNS. The subperineurial glial cells are thin, highly interdigitated cells with epithelial character. The establishment of extensive septate junctions between these cells is crucial for the prevention of uncontrolled paracellular leakage of ions and solutes from the hemolymph into the nervous system. In the absence of septate junctions, macromolecules such as fluorescently labeled dextran can easily cross the blood-brain barrier. To identify additional components of the blood-brain barrier, we followed a genetic approach and injected Texas-Red-conjugated dextran into the hemolymph of embryos homozygous for chromosomal deficiencies. In this way, we identified the 153-aa-large protein Coiled, a new member of the Ly6 (leukocyte antigen 6) family, as being crucially required for septate junction formation and blood-brain barrier integrity. In coiled mutants, the normal distribution of septate junction markers such as NeurexinIV, Coracle, or Discs large is disturbed. EM analyses demonstrated that Coiled is required for the formation of septate junctions. We further show that Coiled is expressed by the subsperineurial glial cells in which it is anchored to the cell membrane via a glycosylphosphatidylinositol anchor and mediates adhesive properties. Clonal rescue studies indicate that the presence of Coiled is required symmetrically on both cells engaged in septate junction formation.
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