ArticleBMC microbiology2011
The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Exploring the reaction dynamics of alanine racemase using serial femtosecond crystallography.Scientific reports · 2024Article
- Article
- Computational and experimental analyses of alanine racemase suggest new avenues for developing allosteric small-molecule antibiotics.Drug development research · 2023Article
- Article
- Purification, Characterization and Inhibition of Alanine Racemase from a Pathogenic Strain ofPolish journal of microbiology · 2019Article
- Knockout of alanine racemase gene attenuates the pathogenicity of Aeromonas hydrophila.BMC microbiology · 2019Article
- Identification of a new alanine racemase inGut pathogens · 2018Article
- Selection and characterization of alanine racemase inhibitors against Aeromonas hydrophila.BMC microbiology · 2017Article
- Article
- The structure of alanine racemase from Acinetobacter baumannii.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Structural and biochemical analyses of alanine racemase from the multidrug-resistant Clostridium difficile strain 630.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Characterization and preliminary mutation analysis of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4.Extremophiles : life under extreme conditions · 2013Article
- Crystallization and preliminary X-ray study of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Streptococcus pneumoniae folate biosynthesis responds to environmental CO2 levels.Journal of bacteriology · 2013Article
- Structure of alanine racemase from Oenococcus oeni with bound pyridoxal 5'-phosphate.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Crystal structures of lysine-preferred racemases, the non-antibiotic selectable markers for transgenic plants.PloS one · 2012Article
- Structural features and kinetic characterization of alanine racemase from Staphylococcus aureus (Mu50).Acta crystallographica. Section D, Biological crystallography · 2012Article
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStreptococcus pneumoniae is a globally important pathogen. The Gram-positive diplococcus is a leading cause of pneumonia, otitis media, bacteremia, and meningitis, and antibiotic resistant strains have become increasingly common over recent years. Alanine racemase is a ubiquitous enzyme among bacteria and provides the essential cell wall precursor, D-alanine. Since it is absent in humans, this enzyme is an attractive target for the development of drugs against S. pneumoniae and other bacterial pathogens.
resultsHere we report the crystal structure of alanine racemase from S. pneumoniae (AlrSP). Crystals diffracted to a resolution of 2.0 Å and belong to the space group P3121 with the unit cell parameters a = b = 119.97 Å, c = 118.10 Å, α = β = 90° and γ = 120°. Structural comparisons show that AlrSP shares both an overall fold and key active site residues with other bacterial alanine racemases. The active site cavity is similar to other Gram positive alanine racemases, featuring a restricted but conserved entryway.
conclusionsWe have solved the structure of AlrSP, an essential step towards the development of an accurate pharmacophore model of the enzyme, and an important contribution towards our on-going alanine racemase structure-based drug design project. We have identified three regions on the enzyme that could be targeted for inhibitor design, the active site, the dimer interface, and the active site entryway.
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