ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2011
Nfatc2 is a primary response gene of Nell-1 regulating chondrogenesis in ATDC5 cells.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
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- Role of NEL‑like molecule‑1 in osteogenesis/chondrogenesis (Review).International journal of molecular medicine · 2025Review
- Genome-Wide Association Studies for Body Conformation Traits in Korean Holstein Population.Animals : an open access journal from MDPI · 2023Article
- Single-cell and spatial transcriptomics reveal changes in cell heterogeneity during progression of human tendinopathy.BMC biology · 2023Article
- Exploring the growth trait molecular markers in two sheep breeds based on Genome-wide association analysis.PloS one · 2023Article
- [Metformin and lipopolysaccharide regulate transcription ofNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2022Article
- Endogenous CCN family member WISP1 inhibits trauma-induced heterotopic ossification.JCI insight · 2020Article
- Peroxisome Proliferator-Activated Receptor-γ Knockdown Impairs Bone Morphogenetic Protein-2-Induced Critical-Size Bone Defect Repair.The American journal of pathology · 2019Article
- ADAM10 modulates SOX9 expressionRSC advances · 2018Article
- Neurexin Superfamily Cell Membrane Receptor Contactin-Associated Protein Like-4 (Cntnap4) Is Involved in Neural EGFL-Like 1 (Nell-1)-Responsive Osteogenesis.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2018Article
- Nfatc1 Is a Functional Transcriptional Factor Mediating Nell-1-Induced Runx3 Upregulation in Chondrocytes.International journal of molecular sciences · 2018Article
- NELL-1 induces Sca-1+ mesenchymal progenitor cell expansion in models of bone maintenance and repair.JCI insight · 2017Article
- Nerve growth factor from Chinese cobra venom stimulates chondrogenic differentiation of mesenchymal stem cells.Cell death & disease · 2017Article
- Neural EGFL-Like 1 Is a Downstream Regulator of Runt-Related Transcription Factor 2 in Chondrogenic Differentiation and Maturation.The American journal of pathology · 2017Article
- Novel Wnt Regulator NEL-Like Molecule-1 Antagonizes Adipogenesis and Augments Osteogenesis Induced by Bone Morphogenetic Protein 2.The American journal of pathology · 2016Article
- Efficacy of Intraperitoneal Administration of PEGylated NELL-1 for Bone Formation.BioResearch open access · 2016Article
- Pharmacokinetics and osteogenic potential of PEGylated NELL-1 in vivo after systemic administration.Biomaterials · 2015Article
- BMP2-induced inflammation can be suppressed by the osteoinductive growth factor NELL-1.Tissue engineering. Part A · 2013Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Nell-1 is a growth factor required for normal skeletal development and expression of extracellular matrix proteins required for bone and cartilage cell differentiation. We identified the transcription factor nuclear factor of activated T cells (Nfatc2) as a primary response gene of Nell-1 through a microarray screen, with validation using real-time polymerase chain reaction (PCR). We investigated the effects of recombinant Nell-1 protein on the chondrogenic cell line ATDC5 and primary mouse chondrocytes. The osteochondral transcription factor Runx2 was investigated as a possible intermediary between Nell-1 and Nfatc2 using adenoviral overexpression of wild-type and dominant-negative Runx2. Nell-1 transiently induced both transcription and translation of Nfatc2, an effect inhibited by transduction of dominant-negative Runx2, suggesting that Runx2 was necessary for Nfatc2 induction. Differentiation assays revealed inhibitory effects of Nell-1 on ATDC5 cells. Although proliferation was unaffected, expression of chondrocyte-specific genes was decreased, and cartilage nodule formation and proteoglycan accumulation were suppressed. siRNA knockdown of Nfatc2 significantly reversed these inhibitory effects. To elucidate the relationship between Nell-1, Runx2, and Nfatc2 in vivo, their presence and distribution were visualized in femurs of wild-type and Nell1-deficient mice at both neonatal and various developmental stages using immunohistochemistry. All three proteins colocalized in the perichondrium of wild-type femurs but stained weakly or were completely absent in Nell1-deficient femurs at neonatal stages. Thus Nfatc2 likely plays an important role in Nell-1-mediated osteochondral differentiation in vitro and in vivo. To our knowledge, this is the first demonstration that Nfatc2 is a primary response gene of Nell-1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.