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ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2011

High-throughput SNP discovery and genotyping in durum wheat (Triticum durum Desf.).

Daniele Trebbi, Marco Maccaferri, Peter de Heer, Anker Sørensen, Silvia Giuliani, Silvio Salvi, Maria Corinna Sanguineti, Andrea Massi, Edwin Andries Gerard van der Vossen, Roberto Tuberosa

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Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Genetic analysis and mapping of adult plant resistance loci to leaf rust in durum wheat cultivar Bairds.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017
    Article
  13. Article
  14. Development of a Genetic Map for Onion (Frontiers in plant science · 2017
    Article
  15. Article
  16. Article
  17. TaXA21-A1 on chromosome 5AL is associated with resistance to multiple pests in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016
    Article
  18. Genome-wide linkage mapping of flour color-related traits and polyphenol oxidase activity in common wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016
    Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Daniele TrebbiKeygene NV, Applied Research, Agro Business Park 90, 6708, PW, Wageningen, The Netherlands. trebbida@msu.edu
Marco Maccaferri
Peter de Heer
Anker Sørensen
Silvia Giuliani
Silvio Salvi
Maria Corinna Sanguineti
Andrea Massi
Edwin Andries Gerard van der Vossen
Roberto Tuberosa

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe the application of complexity reduction of polymorphic sequences (CRoPS(®)) technology for the discovery of SNP markers in tetraploid durum wheat (Triticum durum Desf.). A next-generation sequencing experiment was carried out on reduced representation libraries obtained from four durum cultivars. SNP validation and minor allele frequency (MAF) estimate were carried out on a panel of 12 cultivars, and the feasibility of genotyping these SNPs in segregating populations was tested using the Illumina Golden Gate (GG) technology. A total of 2,659 SNPs were identified on 1,206 consensus sequences. Among the 768 SNPs that were chosen irrespective of their genomic repetitiveness level and assayed on the Illumina BeadExpress genotyping system, 275 (35.8%) SNPs matched the expected genotypes observed in the SNP discovery phase. MAF data indicated that the overall SNP informativeness was high: a total of 196 (71.3%) SNPs had MAF >0.2, of which 76 (27.6%) showed MAF >0.4. Of these SNPs, 157 were mapped in one of two mapping populations (Meridiano × Claudio and Colosseo × Lloyd) and integrated into a common genetic map. Despite the relatively low genotyping efficiency of the GG assay, the validated CRoPS-derived SNPs showed valuable features for genomics and breeding applications such as a uniform distribution across the wheat genome, a prevailing single-locus codominant nature and a high polymorphism. Here, we report a new set of 275 highly robust genome-wide Triticum SNPs that are readily available for breeding purposes.

Indexed as

Genome, PlantGenotypePolymorphism, Single NucleotideBreedingChromosome MappingCrosses, GeneticDNA, PlantGene FrequencyGenetic MarkersGenotyping TechniquesHigh-Throughput Nucleotide SequencingSequence Analysis, DNATetraploidyTriticumDNA, PlantGenetic Markers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.