ArticleJournal of cell science2011
Actin behavior in bulk cytoplasm is cell cycle regulated in early vertebrate embryos.
Article in Journal of cell science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 82 citations in OpenAlex.
- A mechanical ratchet drives unilateral cytokinesis.Nature · 2026Article
- Mechanical self-organization of the actin cytoskeleton in artificial cells.Biophysics and physicobiology · 2026Article
- Conserved nucleocytoplasmic density homeostasis drives cellular organization across eukaryotes.Nature communications · 2025Article
- Cytoplasmic flow is a cell size sensor that scales anaphase.Nature cell biology · 2025Article
- Actin Takes the Steering Wheel: A New Mitotic Safeguard in the Early Embryo.Research (Washington, D.C.) · 2025Article
- Density-dependent flow generation in active cytoskeletal fluids.Scientific reports · 2024Article
- Collective effects of cell cleavage dynamics.Frontiers in cell and developmental biology · 2024Review
- Unveiling the physics underlying symmetry breaking of the actin cytoskeleton: An artificial cell-based approach.Biophysics and physicobiology · 2023Article
- Aurora kinases: Generators of spatial control during mitosis.Frontiers in cell and developmental biology · 2023Review
- Cytoplasmic organization promotes protein diffusion in Xenopus extracts.Nature communications · 2022Article
- Dynein-dependent collection of membranes defines the architecture and position of microtubule asters in isolated, geometrically confined volumes of cell-free extracts.Molecular biology of the cell · 2022Article
- Geometric trade-off between contractile force and viscous drag determines the actomyosin-based motility of a cell-sized droplet.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- DNA Conformation Dictates Strength and Flocculation in DNA-Microtubule Composites.ACS macro letters · 2021Article
- Review
- Cell cycle control during early embryogenesis.Development (Cambridge, England) · 2021Article
- Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization.Journal of visualized experiments : JoVE · 2021Article
- Roadmap for the multiscale coupling of biochemical and mechanical signals during development.Physical biology · 2021Review
- Affinity Purification of Label-free Tubulins fromSTAR protocols · 2020Article
- Article
- Tug-of-war between actomyosin-driven antagonistic forces determines the positioning symmetry in cell-sized confinement.Nature communications · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
The mechanical properties of cells change as they proceed through the cell cycle, primarily owing to regulation of actin and myosin II. Most models for cell mechanics focus on actomyosin in the cortex and ignore possible roles in bulk cytoplasm. We explored cell cycle regulation of bulk cytoplasmic actomyosin in Xenopus egg extracts, which is almost undiluted cytoplasm from unfertilized eggs. We observed dramatic gelation-contraction of actomyosin in mitotic (M phase) extract where Cdk1 activity is high, but not in interphase (I-phase) extract. In spread droplets, M-phase extract exhibited regular, periodic pulses of gelation-contraction a few minutes apart that continued for many minutes. Comparing actin nucleation, disassembly and myosin II activity between M-phase and I-phase extracts, we conclude that regulation of nucleation is likely to be the most important for cell cycle regulation. We then imaged F-actin in early zebrafish blastomeres using a GFP-Utrophin probe. Polymerization in bulk cytoplasm around vesicles increased dramatically during mitosis, consistent with enhanced nucleation. We conclude that F-actin polymerization in bulk cytoplasm is cell cycle regulated in early vertebrate embryos and discuss possible biological functions of this regulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.