ArticleMethods in enzymology2009
Fluorescence spectroscopy in thermodynamic and kinetic analysis of pH-dependent membrane protein insertion.
Article in Methods in enzymology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Membrane interactions of apoptotic inhibitor Bcl-xL: What can be learned using fluorescence spectroscopy.BBA advances · 2023Article
- Design and Characterization of Myristoylated and Non-Myristoylated Peptides Effective againstInternational journal of molecular sciences · 2022Article
- Advances in monitoring and control of refolding kinetics combining PAT and modeling.Applied microbiology and biotechnology · 2021Review
- Conformational switching, refolding and membrane insertion of the diphtheria toxin translocation domain.Methods in enzymology · 2021Article
- Article
- Trematocine, a Novel Antimicrobial Peptide from the Antarctic FishAntibiotics (Basel, Switzerland) · 2020Article
- Design and characterization of chionodracine-derived antimicrobial peptides with enhanced activity against drug-resistant human pathogens.RSC advances · 2018Article
- Kinetics of peptide folding in lipid membranes.Biopolymers · 2015Review
- Membrane Association of the Diphtheria Toxin Translocation Domain Studied by Coarse-Grained Simulations and Experiment.The Journal of membrane biology · 2015Article
- Thermodynamics of Membrane Insertion and Refolding of the Diphtheria Toxin T-Domain.The Journal of membrane biology · 2015Article
- Role of acidic residues in helices TH8-TH9 in membrane interactions of the diphtheria toxin T domain.Toxins · 2015Article
- Targeting acidity in diseased tissues: mechanism and applications of the membrane-inserting peptide, pHLIP.Archives of biochemistry and biophysics · 2015Article
- Comparison of membrane insertion pathways of the apoptotic regulator Bcl-xL and the diphtheria toxin translocation domain.Biochemistry · 2013Article
- Thermodynamic measurements of bilayer insertion of a single transmembrane helix chaperoned by fluorinated surfactants.Journal of molecular biology · 2012Article
- Lipid-modulation of membrane insertion and refolding of the apoptotic inhibitor Bcl-xL.Biochimica et biophysica acta. Proteins and proteomicsArticle
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Abstract
Experimental determination of the free energy stabilizing the structure of membrane proteins in their native lipid environment is undermined by a lack of appropriate methods and suitable model systems. Here, we demonstrate how fluorescence correlation spectroscopy can be used to characterize thermodynamics of pH-triggered bilayer insertion of nonconstitutive membrane proteins (e.g., bacterial toxins, colicins). The experimental design is guided by the appropriate thermodynamic scheme which considers two independent processes: pH-dependent formation of a membrane-competent form and its insertion into the lipid bilayer. Measurements of a model protein annexin B12 under conditions of lipid saturation demonstrate that protonation leading to the formation of the membrane-competent state occurs near membrane interface. Lipid titration experiments demonstrate that the free energy of transfer to the intermediate interfacial state is especially favorable, while the free energy of final insertion is modulated by interplay of hydrophobic and electrostatic interactions on the bilayer interface. The general principles of kinetic measurements along the insertion pathway containing interfacial intermediate are discussed and practical examples emphasizing appropriate fitting and normalization procedures are presented.
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