ArticleJournal of laboratory automation2011
Automation of three-dimensional cell culture in arrayed microfluidic devices.
Article in Journal of laboratory automation, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay.Molecules (Basel, Switzerland) · 2021Article
- Hydrogels as artificial matrices for cell seeding in microfluidic devices.RSC advances · 2020Review
- On-chip 3D neuromuscular model for drug screening and precision medicine in neuromuscular disease.Nature protocols · 2020Article
- Engineering a Model to Study Viral Infections: Bioprinting, Microfluidics, and Organoids to Defeat Coronavirus Disease 2019 (COVID-19).International journal of bioprinting · 2020Review
- Integrating 3D Cell Culture of PC12 Cells with Microchip-Based Electrochemical Detection.Analytical methods : advancing methods and applications · 2019Article
- Adaptation of a Simple Microfluidic Platform for High-Dimensional Quantitative Morphological Analysis of Human Mesenchymal Stromal Cells on Polystyrene-Based Substrates.SLAS technology · 2017Article
- Multiwell capillarity-based microfluidic device for the study of 3D tumour tissue-2D endothelium interactions and drug screening in co-culture models.Scientific reports · 2017Article
- Cell Microarray Technologies for High-Throughput Cell-Based Biosensors.Sensors (Basel, Switzerland) · 2017Review
- Single cell functional analysis of multiple myeloma cell populations correlates with diffusion profiles in static microfluidic coculture systems.Biomicrofluidics · 2016Article
- Microfluidic cell chips for high-throughput drug screening.Bioanalysis · 2016Review
- Microfluidics-based 3D cell culture models: Utility in novel drug discovery and delivery research.Bioengineering & translational medicine · 2016Review
- Article
- Microscale screening systems for 3D cellular microenvironments: platforms, advances, and challenges.Cellular and molecular life sciences : CMLS · 2015Review
- Platform for high-throughput testing of the effect of soluble compounds on 3D cell cultures.Analytical chemistry · 2013Article
- Building risk-on-a-chip models to improve breast cancer risk assessment and prevention.Integrative biology : quantitative biosciences from nano to macro · 2013Review
- Microengineered tumor models: insights & opportunities from a physical sciences-oncology perspective.Biomedical microdevices · 2013Review
- Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models.PloS one · 2013Article
- Review
- A practical method for patterning lumens through ECM hydrogels via viscous finger patterning.Journal of laboratory automation · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The increasing interest in studying the interactions between cells and the extracellular matrix (ECM) has created a need for high throughput low-cost three-dimensional (3D) culture systems. The recent development of tubeless microfluidics via passive pumping provides a high throughput microchannel culture platform compatible with existing high throughput infrastructures (e.g., automated liquid handlers). Here, we build on a previously reported high throughput two-dimensional system to create a robust automated system for 3D culture. Operational controls including temperature and sample handling have been characterized and automated. Human mammary fibroblasts (HMFs) suspended in type I collagen are loaded and cultured in microchannel arrays and used to optimize the system operational parameters. A Peltier cooler maintains the collagen as a liquid at 4 °C during cell seeding, followed by polymerization at 37 °C. Optimization of this platform is discussed (e.g., controlling collagen contraction, increasing cell viability, preventing the removal of microchannel contents), and 3D distribution of HMFs is examined by fluorescent microscopy. Finally, we validate the platform by automating a previously developed 3D breast carcinoma coculture assay. The platform allows more efficient 3D culture experiments and lays the foundation for high throughput studies of cell-ECM interactions.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.