ArticleThe Journal of biological chemistry2011
Autoacetylation of the histone acetyltransferase Rtt109.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 66 citations in OpenAlex.
- Dynamic global acetylation remodeling during the yeast heat shock response.Genome biology · 2026Article
- A chemical catalyst enabling histone acylation with endogenous acyl-CoA.Nature communications · 2023Article
- Histone post-translational modification and the DNA damage response.Genes & diseases · 2023Review
- Suppressor mutations that make the essential transcription factor Spn1/Iws1 dispensable in Saccharomyces cerevisiae.Genetics · 2022Article
- Phosphorylation and acetylation of mitochondrial transcription factor A promote transcription processivity without compromising initiation or DNA compaction.The Journal of biological chemistry · 2022Article
- Protein Acetyltransferases Mediate Bacterial Adaptation to a Diverse Environment.Journal of bacteriology · 2021Review
- Review
- Review
- Catalysis by protein acetyltransferase Gcn5.Biochimica et biophysica acta. Gene regulatory mechanisms · 2021Review
- Cisplatin inhibits SIRT3-deacetylation MTHFD2 to disturb cellular redox balance in colorectal cancer cell.Cell death & disease · 2020Article
- Mechanism of activation for the sirtuin 6 protein deacylase.The Journal of biological chemistry · 2020Article
- Characterizing Lysine Acetylation of Isocitrate Dehydrogenase in Escherichia coli.Journal of molecular biology · 2018Article
- Lysine Acetylation Goes Global: From Epigenetics to Metabolism and Therapeutics.Chemical reviews · 2018Review
- Review
- A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli.Journal of visualized experiments : JoVE · 2017Article
- KATapulting toward Pluripotency and Cancer.Journal of molecular biology · 2017Review
- Studying the Lysine Acetylation of Malate Dehydrogenase.Journal of molecular biology · 2017Article
- YopJ Family Effectors Promote Bacterial Infection through a Unique Acetyltransferase Activity.Microbiology and molecular biology reviews : MMBR · 2016Review
- Structural and Functional Role of Acetyltransferase hMOF K274 Autoacetylation.The Journal of biological chemistry · 2016Article
- Molecular Basis for Histone Acetyltransferase Regulation by Binding Partners, Associated Domains, and Autoacetylation.ACS chemical biology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Rtt109 is a yeast histone acetyltransferase (HAT) that associates with histone chaperones Asf1 and Vps75 to acetylate H3K56, H3K9, and H3K27 and is important in DNA replication and maintaining genomic integrity. Recently, mass spectrometry and structural studies of Rtt109 have shown that active site residue Lys-290 is acetylated. However, the functional role of this modification and how the acetyl group is added to Lys-290 was unclear. Here, we examined the mechanism of Lys-290 acetylation and found that Rtt109 catalyzes intramolecular autoacetylation of Lys-290 ∼200-times slower than H3 acetylation. Deacetylated Rtt109 was prepared by reacting with a sirtuin protein deacetylase, producing an enzyme with negligible HAT activity. Autoacetylation of Rtt109 restored full HAT activity, indicating that autoacetylation is necessary for HAT activity and is a fully reversible process. To dissect the mechanism of activation, biochemical, and kinetic analyses were performed with Lys-290 variants of the Rtt109-Vps75 complex. We found that autoacetylation of Lys-290 increases the binding affinity for acetyl-CoA and enhances the rate of acetyl-transfer onto histone substrates. This study represents the first detailed investigation of a HAT enzyme regulated by single-site intramolecular autoacetylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.