ArticleThe Journal of biological chemistry2011
Insights into the composition and assembly of the membrane arm of plant complex I through analysis of subcomplexes in Arabidopsis mutant lines.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- PCIS1 is an essential factor in mitochondrial RNA splicing and complex I biogenesis, with distinct effects in null and downregulated mutants.Plant cell reports · 2026Article
- Disturbed RNA editing in MORF3-deficient Arabidopsis mitochondria leads to impaired assembly of complex I.Plant physiology · 2025Article
- C1-FDX is required for the assembly of mitochondrial complex I and subcomplexes of complex V in Arabidopsis.PLoS genetics · 2024Article
- Plant supercomplex I + III2 structure and function: implications for the growing field.Biochemical Society transactions · 2024Review
- PPR596 Is Required forInternational journal of molecular sciences · 2024Article
- Alteration of Mitochondrial Transcript Expression inInternational journal of molecular sciences · 2023Article
- FTSH PROTEASE 3 facilitates Complex I degradation through a direct interaction with the Complex I subunit PSST.The Plant cell · 2023Article
- The biogenesis and regulation of the plant oxidative phosphorylation system.Plant physiology · 2023Review
- Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis.Plant physiology · 2023Article
- Plant-specific features of respiratory supercomplex I + IIINature plants · 2023Article
- Subunits E-F-G of E. coli Complex I can form an active complex when expressed alone, but in time-delayed assembly co-expression of B-CD-E-F-G is optimal.Biochimica et biophysica acta. Bioenergetics · 2022Article
- Review
- Protein lipoylation in mitochondria requires Fe-S cluster assembly factors NFU4 and NFU5.Plant physiology · 2022Article
- High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The function of glutaredoxin GRXS15 is required for lipoyl-dependent dehydrogenases in mitochondria.Plant physiology · 2021Article
- The mitochondrial AAA protease FTSH3 regulates Complex I abundance by promoting its disassembly.Plant physiology · 2021Article
- Molecular cloning and transcriptional regulation of two γ-carbonic anhydrase genes in the green macroalga Ulva prolifera.Genetica · 2021Article
- Analysis of the assembly pathway for membrane subunits of Complex I reveals that subunit L (ND5) can assemble last inBBA advances · 2021Article
- Article
- Mitochondrial Pentatricopeptide Repeat Protein, EMB2794, Plays a Pivotal Role in NADH Dehydrogenase Subunit nad2 mRNA Maturation in Arabidopsis thaliana.Plant & cell physiology · 2020Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NADH-ubiquinone oxidoreductase (Complex I, EC 1.6.5.3) is the largest complex of the mitochondrial respiratory chain. In eukaryotes, it is composed of more than 40 subunits that are encoded by both the nuclear and mitochondrial genomes. Plant Complex I differs from the enzyme described in other eukaryotes, most notably due to the large number of plant-specific subunits in the membrane arm of the complex. The elucidation of the assembly pathway of Complex I has been a long-standing research aim in cellular biochemistry. We report the study of Arabidopsis mutants in Complex I subunits using a combination of Blue-Native PAGE and immunodetection to identify stable subcomplexes containing Complex I components, along with mass spectrometry analysis of Complex I components in membrane fractions and two-dimensional diagonal Tricine SDS-PAGE to study the composition of the largest subcomplex. Four subcomplexes of the membrane arm of Complex I with apparent molecular masses of 200, 400, 450, and 650 kDa were observed. We propose a working model for the assembly of the membrane arm of Complex I in plants and assign putative roles during the assembly process for two of the subunits studied.
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