ArticleProceedings of the National Academy of Sciences of the United States of America2011
Forces guiding assembly of light-harvesting complex 2 in native membranes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Single Molecule Force Spectroscopy to Probe Intermediates and Energetics of Membrane Protein Folding.Chemical reviews · 2026Review
- New insights into the structure and dynamics of the TOM complex in mitochondria.Biochemical Society transactions · 2024Review
- Native architecture and acclimation of photosynthetic membranes in a fast-growing cyanobacterium.Plant physiology · 2022Article
- Structural basis for the assembly and quinone transport mechanisms of the dimeric photosynthetic RC-LH1 supercomplex.Nature communications · 2022Article
- Characterizing the supercomplex association of photosynthetic complexes in cyanobacteria.Royal Society open science · 2021Article
- AlteredeLife · 2020Article
- Structural Visualization of Septum Formation in Staphylococcus warneri Using Atomic Force Microscopy.Journal of bacteriology · 2020Article
- Unfolding pathway and intermolecular interactions of the cytochrome subunit in the bacterial photosynthetic reaction center.Biochimica et biophysica acta. Bioenergetics · 2020Article
- Self-Assembly Stability and Variability of Bacterial Microcompartment Shell Proteins in Response to the Environmental Change.Nanoscale research letters · 2019Article
- Insights into the Origin of Distinct Medin Fibril Morphologies Induced by Incubation Conditions and Seeding.International journal of molecular sciences · 2018Article
- High temperature limit of photosynthetic excitons.Nature communications · 2018Article
- Engineering and Modulating Functional Cyanobacterial COFrontiers in plant science · 2018Article
- Direct characterization of the native structure and mechanics of cyanobacterial carboxysomes.Nanoscale · 2017Article
- The non-bilayer lipid MGDG stabilizes the major light-harvesting complex (LHCII) against unfolding.Scientific reports · 2017Article
- Visualization of Bacterial Microcompartment Facet Assembly Using High-Speed Atomic Force Microscopy.Nano letters · 2016Article
- Distribution and dynamics of electron transport complexes in cyanobacterial thylakoid membranes.Biochimica et biophysica acta · 2016Review
- Oxidative Stress Alters the Morphology and Toxicity of Aortic Medial Amyloid.Biophysical journal · 2015Article
- Principles of light harvesting from single photosynthetic complexes.Interface focus · 2015Review
- Aberrant assembly complexes of the reaction center light-harvesting 1 PufX (RC-LH1-PufX) core complex of Rhodobacter sphaeroides imaged by atomic force microscopy.The Journal of biological chemistry · 2014Article
- Phycobilisome Mobility and Its Role in the Regulation of Light Harvesting in Red Algae.Plant physiology · 2014Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interaction forces of membrane protein subunits are of importance in their structure, assembly, membrane insertion, and function. In biological membranes, and in the photosynthetic apparatus as a paradigm, membrane proteins fulfill their function by ensemble actions integrating a tight assembly of several proteins. In the bacterial photosynthetic apparatus light-harvesting complexes 2 (LH2) transfer light energy to neighboring tightly associated core complexes, constituted of light-harvesting complexes 1 (LH1) and reaction centers (RC). While the architecture of the photosynthetic unit has been described, the forces and energies assuring the structural and functional integrity of LH2, the assembly of LH2 complexes, and how LH2 interact with the other proteins in the supramolecular architecture are still unknown. Here we investigate the molecular forces of the bacterial LH2 within the native photosynthetic membrane using atomic force microscopy single-molecule imaging and force measurement in combination. The binding between LH2 subunits is fairly weak, of the order of k(B)T, indicating the importance of LH2 ring architecture. In contrast LH2 subunits are solid with a free energy difference of 90 k(B)T between folded and unfolded states. Subunit α-helices unfold either in one-step, α- and β-polypeptides unfold together, or sequentially. The unfolding force of transmembrane helices is approximately 150 pN. In the two-step unfolding process, the β-polypeptide is stabilized by the molecular environment in the membrane. Hence, intermolecular forces influence the structural and functional integrity of LH2.
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