ArticleProceedings of the National Academy of Sciences of the United States of America2011
Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin:NADP+-oxidoreductase (FNR) enzymes in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 186 citations in OpenAlex.
- Article
- Structural proteomics reveals the functional docking interface of ferredoxin-NADPThe Plant journal : for cell and molecular biology · 2026Article
- Molecular design principles for Photosystem I-based biohybrid solar fuel catalysts.bioRxiv : the preprint server for biology · 2026Article
- Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis.Applied microbiology and biotechnology · 2026Article
- Artificial mediators, protein carriers, and catalyst architecture in C1 bioconversion.Frontiers in bioengineering and biotechnology · 2026Review
- Outlook on Synthetic Biology-Driven Hydrogen Production: Lessons from Algal Photosynthesis Applied to Cyanobacteria.Energy & fuels : an American Chemical Society journal · 2025Review
- Light-Driven HPlants (Basel, Switzerland) · 2024Review
- Regulation of hydrogen rich water on strawberry seedlings and root endophytic bacteria under salt stress.Frontiers in plant science · 2024Article
- Artificial photosynthetic cells with biotic-abiotic hybrid energy modules for customized CONature communications · 2023Article
- Scaling-up and proteomic analysis reveals photosynthetic and metabolic insights toward prolonged HPhotosynthesis research · 2022Article
- Synthetic biology for improved hydrogen production in Chlamydomonas reinhardtii.Microbial biotechnology · 2022Review
- Bioconversion of CO to formate by artificially designed carbon monoxide:formate oxidoreductase in hyperthermophilic archaea.Communications biology · 2022Article
- Sustainability of in vitro light-dependent NADPH generation by the thylakoid membrane of Synechocystis sp. PCC6803.Microbial cell factories · 2022Article
- Photosystem I light-harvesting proteins regulate photosynthetic electron transfer and hydrogen production.Plant physiology · 2022Article
- Exploiting photosynthesis-driven P450 activity to produce indican in tobacco chloroplasts.Frontiers in plant science · 2022Article
- Improved photosynthetic capacity and photosystem I oxidation via heterologous metabolism engineering in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Investigation of the Ferredoxin's Influence on the Anaerobic and Aerobic, Enzymatic HFrontiers in bioengineering and biotechnology · 2021Article
- Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production.Nature communications · 2020Article
- Tweaking Photosynthesis: FNR-TROL Interaction as Potential Target for Crop Fortification.Frontiers in plant science · 2020Article
- Ferredoxin5 Deletion Affects Metabolism of Algae during the Different Phases of Sulfur Deprivation.Plant physiology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photosynthetic water splitting, coupled to hydrogenase-catalyzed hydrogen production, is considered a promising clean, renewable source of energy. It is widely accepted that the oxygen sensitivity of hydrogen production, combined with competition between hydrogenases and NADPH-dependent carbon dioxide fixation are the main limitations for its commercialization. Here we provide evidence that, under the anaerobic conditions that support hydrogen production, there is a significant loss of photosynthetic electrons toward NADPH production in vitro. To elucidate the basis for competition, we bioengineered a ferredoxin-hydrogenase fusion and characterized hydrogen production kinetics in the presence of Fd, ferredoxin:NADP(+)-oxidoreductase (FNR), and NADP(+). Replacing the hydrogenase with a ferredoxin-hydrogenase fusion switched the bias of electron transfer from FNR to hydrogenase and resulted in an increased rate of hydrogen photoproduction. These results suggest a new direction for improvement of biohydrogen production and a means to further resolve the mechanisms that control partitioning of photosynthetic electron transport.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.