ArticleNaunyn-Schmiedeberg's archives of pharmacology1990
Direct inhibition of chicken gizzard smooth muscle contractile apparatus by caffeine.
Article in Naunyn-Schmiedeberg's archives of pharmacology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Antispasmodic Activity of Light-Roasted Coffee Extract and Its Potential Use in Gastrointestinal Motility Disorders.Foods (Basel, Switzerland) · 2024Article
- Characterisation of Lipoma-Preferred Partner as a Novel Mechanotransducer in Vascular Smooth Muscle Cells.Cells · 2023Article
- Coffee and Endothelial Function: A Coffee Paradox?Nutrients · 2019Review
- Pu-Erh Tea Relaxes the Thoracic Aorta of Rats by Reducing Intracellular Calcium.Frontiers in pharmacology · 2019Article
- Phenylephrine, a common cold remedy active ingredient, suppresses uterine contractions through cAMP signalling.Scientific reports · 2018Article
- Endothelial Nitric Oxide Mediates Caffeine Antagonism of Alcohol-Induced Cerebral Artery Constriction.The Journal of pharmacology and experimental therapeutics · 2016Article
- Caffeine relaxes smooth muscle through actin depolymerization.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Caffeine's Vascular Mechanisms of Action.International journal of vascular medicine · 2010Article
- High K+-induced contraction requires depolarization-induced Ca2+ release from internal stores in rat gut smooth muscle.Acta pharmacologica Sinica · 2009Article
- Possible mechanism of the potent vasoconstrictor actions of ryanodine on femoral arteries from spontaneously hypertensive rats.British journal of pharmacology · 1996Article
- Effects of phenol on vascular smooth muscle in rabbit mesenteric resistance arteries.Journal of anesthesia · 1996Article
- Focal [Ca2+]i increases detected by aequorin but not by fura-2 in histamine- and caffeine-stimulated swine carotid artery.The Journal of physiology · 1995Article
- Caffeine-induced release and reuptake of Ca2+ by Ca2+ stores in myocytes from guinea-pig urinary bladder.The Journal of physiology · 1992Article
- Mechanisms of caffeine-induced contraction and relaxation of rat aortic smooth muscle.The Journal of physiology · 1992Article
- Cyclic AMP-mediated regulation of excitation-contraction coupling in canine gastric smooth muscle.The Journal of physiology · 1992Article
- Mechanisms controlling caffeine-induced relaxation of coronary artery of the pig.British journal of pharmacology · 1991Article
- Compartmentation of ATP synthesis and utilization in smooth muscle: roles of aerobic glycolysis and creatine kinase.Molecular and cellular biochemistryReview
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism of the inhibitory effect of caffeine on smooth muscle contraction was examined using chicken gizzard. Caffeine (0.1-5 mmol/l) inhibited the KCl-induced contraction of the muscle with an IC50 of 1.1 mmol/l. Forskolin (0.01-10 mumol/l) also inhibited KCl-induced contraction. The inhibitory effect of caffeine was potentiated by a low concentration of forskolin (0.3 mumol/l) and the inhibitory effect of forskolin was potentiated by a low concentration of caffeine (0.1 mmol/l). Although caffeine and forskolin increased tissue cyclic AMP levels, caffeine inhibited the KCl-induced contraction more strongly than forskolin at a given cyclic AMP level. Caffeine (1-40 mmol/l) inhibited the contractions induced by 3 mumol/l Ca2+ in Triton X-100-permeabilized muscle. Caffeine (1-40 mmol/l) inhibited the phosphorylation of 20 kDa myosin light chain (MLC) in native actomyosin preparation and the inhibition was enhanced by decreasing the ATP concentration in the reaction medium. Calmodulin (CaM) activity, as monitored by Ca2+/CaM-dependent erythrocyte membrane (Ca2+ + Mg2+)-ATPase, was not affected by 20 mmol/l caffeine. Time-dependent dephosphorylation of MLC upon removal of Ca2+, an indicator of phosphate activity, was not affected by caffeine. Caffeine also inhibited the Ca2(+)-independent contraction in thiophosphorylated permeabilized muscle. These results indicate that caffeine inhibits smooth muscle contraction by a direct inhibition of MLC kinase and actin-myosin interaction. A part of the inhibitory effect may be mediated by cyclic AMP-dependent mechanism(s).
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