ReviewMolecular biotechnology2011
Deciphering enzyme function using peptide arrays.
Review in Molecular biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- Tumor phosphatidylinositol-3-kinase signaling and development of metastatic disease in locally advanced rectal cancer.PloS one · 2012Trial
- Uncovering Enzyme-Specific Post-Translational Modifications: An Overview of Current Methods.Proteomes · 2025Review
- Streamlined Identification of Metallopeptides for Intracellular Catalysis Using Positionally Addressable Combinatorial Libraries.ACS catalysis · 2025Article
- Peptide Arrays: Development and Application.Analytical chemistry · 2018Review
- Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities.Methods in enzymology · 2018Article
- Identifying Dysregulated Epigenetic Enzyme Activity in Castrate-Resistant Prostate Cancer Development.ACS chemical biology · 2017Article
- Discovery of a microbial transglutaminase enabling highly site-specific labeling of proteins.The Journal of biological chemistry · 2017Article
- Identification of functional metabolic biomarkers from lung cancer patient serum using PEP technology.Biomarker research · 2016Article
- Bead Based Proteome Enrichment Enhances Features of the Protein Elution Plate (PEP) for Functional Proteomic Profiling.Proteomes · 2015Article
- Identification of multiple metabolic enzymes from mice cochleae tissue using a novel functional proteomics technology.PloS one · 2015Article
- Application of a peptide-based assay to characterize inhibitors targeting protein kinases from yeast.Current genetics · 2014Article
- Article
- Development of the schedule for multiple parallel "difficult" Peptide synthesis on pins.International journal of peptides · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last years. This technology reduces set-up time for HTS assays and allows the identification of downstream targets. Moreover, peptide microarrays enable optimisation of enzyme substrates. Focus of this review is on assay principles for measuring activities of kinases, phosphatases or proteases and on substrate identification/optimisation for kinases. Additionally, several examples for reliable identification of substrates for lysine methyl-transferases, histone deacetylases and SUMO-transferases are given. Finally, use of high-density peptide microarrays for the simultaneous profiling of kinase activities in complex biological samples like cell lysates or lysates of complete organisms is described. All published examples of peptide arrays used for enzyme profiling are summarised comprehensively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.