ArticleJournal of materials science. Materials in medicine2011
Functionalisation of PLLA nanofiber scaffolds using a possible cooperative effect between collagen type I and BMP-2: impact on growth and osteogenic differentiation of human mesenchymal stem cells.
Article in Journal of materials science. Materials in medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Direct incorporation of mesenchymal stem cells into a Nanofiber scaffold - in vitro and in vivo analysis.Scientific reports · 2020Article
- Effects of bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) release from polylactide-poly (ethylene glycol)-polylactide (PELA) microcapsule-based scaffolds on bone.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2017Article
- Osteogenic differentiation of mesenchymal stem cells cultured on PLLA scaffold coated with Wharton's Jelly.EXCLI journal · 2017Article
- Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering.Biomaterials research · 2017Article
- Micro/Nano Multilayered Scaffolds of PLGA and Collagen by Alternately Electrospinning for Bone Tissue Engineering.Nanoscale research letters · 2016Article
- Focal adhesion kinase regulation in stem cell alignment and spreading on nanofibers.Biochemical and biophysical research communications · 2016Article
- MWCNTs enhance hBMSCs spreading but delay their proliferation in the direction of differentiation acceleration.Cell adhesion & migration · 2014Article
- The effect of five proteins on stem cells used for osteoblast differentiation and proliferation: a current review of the literature.Cellular and molecular life sciences : CMLS · 2014Review
- Scaffold design for bone regeneration.Journal of nanoscience and nanotechnology · 2014Review
- Multiwalled carbon nanotubes enhance human bone marrow mesenchymal stem cells' spreading but delay their proliferation in the direction of differentiation acceleration.Cell adhesion & migration · 2014Article
- Rational design of nanofiber scaffolds for orthopedic tissue repair and regeneration.Nanomedicine (London, England) · 2013Review
- Bone formation in rabbit cancellous bone explant culture model is enhanced by mechanical load.Biomedical engineering online · 2013Article
- Functionalisation of PLLA nanofiber scaffolds using a possible cooperative effect between collagen type I and BMP-2: impact on colonization and bone formation in vivo.Journal of materials science. Materials in medicine · 2012Article
- Porous collagen scaffold reinforced with surfaced activated PLLA nanoparticles.TheScientificWorldJournal · 2012Article
- Physical properties and biocompatibility of a core-sheath structure composite scaffold for bone tissue engineering in vitro.Journal of biomedicine & biotechnology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cell differentiation of osteoblasts is triggered by a series of signaling processes including integrin and bone morphogenetic protein (BMP), which therefore act in a cooperative manner. The aim of this study was to analyze whether these processes can be remodeled in an artificial poly-(L)-lactide acid (PLLA) based nanofiber scaffold. Matrices composed of PLLA-collagen type I or BMP-2 incorporated PLLA-collagen type I were seeded with human mesenchymal stem cells (hMSC) and cultivated over a period of 22 days, either under growth or osteoinductive conditions. During the course of culture, gene expression of alkaline phosphatase (ALP), osteocalcin (OC) and collagen I (COL-I) as well as Smad5 and focal adhesion kinase (FAK), two signal transduction molecules involved in BMP-2 or integrin signaling were analyzed. Furthermore, calcium and collagen I deposition, as well as cell densities and proliferation, were determined using fluorescence microscopy. The incorporation of BMP-2 into PLLA-collagen type I nanofibers resulted in a decrease in diameter as well as pore sizes of the scaffold. Mesenchymal stem cells showed better adherence and a reduced proliferation on BMP-containing scaffolds. This was accompanied by an increase in gene expression of ALP, OC and COL-I. Furthermore the presence of BMP-2 resulted in an upregulation of FAK, while collagen had an impact on the gene expression of Smad5. Therefore these different strategies can be combined in order to enhance the osteoblast differentiation of hMSC on PLLA based nanofiber scaffold. By doing this, different signal transduction pathways seem to be up regulated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.