ArticlePloS one2011
Firefly luciferase and RLuc8 exhibit differential sensitivity to oxidative stress in apoptotic cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Development and validation of Mayaro virus with luciferase reporter genes as a tool for antiviral assays.Heliyon · 2024Article
- Recent Applications and Future Perspectives of Chemiluminescent and Bioluminescent Imaging Technologies.Chemical & biomedical imaging · 2023Review
- Bioluminescence-Based Complementation Assay to Correlate Conformational Changes in Membrane-Bound Complexes with Enzymatic Function.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Bioluminescence resonance energy transfer-based imaging of protein-protein interactions in living cells.Nature protocols · 2019Article
- Transgenic Animal Models to Visualize Cancer-Related Cellular Processes by Bioluminescence Imaging.Frontiers in pharmacology · 2019Review
- Integrated omics analyses of retrograde signaling mutant delineate interrelated stress-response strata.The Plant journal : for cell and molecular biology · 2017Article
- Translational Pharmacokinetic-Pharmacodynamic Modeling and Simulation: Optimizing 5-Fluorouracil Dosing in Children With Pediatric Ependymoma.CPT: pharmacometrics & systems pharmacology · 2016Article
- Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag.Journal of bacteriology · 2016Article
- In vivo cell tracking with bioluminescence imaging.Nuclear medicine and molecular imaging · 2015Review
- Noninvasive bioluminescence imaging of α-synuclein oligomerization in mouse brain using split firefly luciferase reporters.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Luciferase does not Alter Metabolism in Cancer Cells.Metabolomics : Official journal of the Metabolomic Society · 2014Article
- Bioluminescence: a versatile technique for imaging cellular and molecular features.MedChemComm · 2014Article
- A genetically encoded IL-1β bioluminescence resonance energy transfer sensor to monitor inflammasome activity.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Approaches to engineer stability of beetle luciferases.Computational and structural biotechnology journal · 2012Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the past decade, firefly Luciferase (fLuc) has been used in a wide range of biological assays, providing insight into gene regulation, protein-protein interactions, cell proliferation, and cell migration. However, it has also been well established that fLuc activity can be highly sensitive to its surrounding environment. In this study, we found that when various cancer cell lines (HeLa, MCF-7, and 293T) stably expressing fLuc were treated with staurosporine (STS), there was a rapid loss in bioluminescence. In contrast, a stable variant of Renilla luciferase (RLuc), RLuc8, exhibited significantly prolonged functionality under the same conditions. To identify the specific underlying mechanism(s) responsible for the disparate sensitivity of RLuc8 and fLuc to cellular stress, we conducted a series of inhibition studies that targeted known intracellular protein degradation/modification pathways associated with cell death. Interestingly, these studies suggested that reactive oxygen species, particularly hydrogen peroxide (H(2)O(2)), was responsible for the diminution of fLuc activity. Consistent with these findings, the direct application of H(2)O(2) to HeLa cells also led to a reduction in fLuc bioluminescence, while H(2)O(2) scavengers stabilized fLuc activity. Comparatively, RLuc8 was far less sensitive to ROS. These observations suggest that fLuc activity can be substantially altered in studies where ROS levels become elevated and can potentially lead to ambiguous or misleading findings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.