ArticleFemale pelvic medicine & reconstructive surgery2010
Repetitive mechanical stretch increases extracellular collagenase activity in vaginal fibroblasts.
Article in Female pelvic medicine & reconstructive surgery, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Age and/or postmenopausal status as risk factors for pelvic organ prolapse development: systematic review with meta-analysis.International urogynecology journal · 2022Pooled it
- Comparison of Culturing Methods of Primary Vaginal Fibroblasts.Urogynecology (Philadelphia, Pa.) · 2025Article
- Genetics of Female Pelvic Organ Prolapse: Up to Date.Biomolecules · 2024Review
- Roles and mechanisms of biomechanical-biochemical coupling in pelvic organ prolapse.Frontiers in medicine · 2024Review
- Molecular Landscape of Pelvic Organ Prolapse Provides Insights into Disease Etiology.International journal of molecular sciences · 2023Article
- Comparison between transumbilical and transvaginal morcellation of a large uterus during single-port-access total laparoscopic hysterectomy.Obstetrics & gynecology science · 2020Article
- Engineering and women's health: a slow start, but gaining momentum.Interface focus · 2019Review
- Pelvic muscles' mechanical response to strains in the absence and presence of pregnancy-induced adaptations in a rat model.American journal of obstetrics and gynecology · 2018Article
- Transplantation of bone marrow-derived mesenchymal stem cells expressing elastin alleviates pelvic floor dysfunction.Stem cell research & therapy · 2016Article
- Vaginal Fibroblastic Cells from Women with Pelvic Organ Prolapse Produce Matrices with Increased Stiffness and Collagen Content.Scientific reports · 2016Article
- Effects of mechanical stretching on the morphology and cytoskeleton of vaginal fibroblasts from women with pelvic organ prolapse.International journal of molecular sciences · 2015Article
- Challenges and future prospects for tissue engineering in female pelvic medicine and reconstructive surgery.Current urology reports · 2014Review
- Inflammatory and tissue remodeling urinary biomarkers before and after mid urethral sling surgery for stress urinary incontinence.The Journal of urology · 2014Article
- Fibroblasts from women with pelvic organ prolapse show differential mechanoresponses depending on surface substrates.International urogynecology journal · 2013Article
- Vaginal childbirth and pelvic floor disorders.Women's health (London, England) · 2013Review
- Pelvic floor disorders following vaginal or cesarean delivery.Current opinion in obstetrics & gynecology · 2012Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
objectivesThe objectives were 1) to determine whether human vaginal fibroblasts are mechanosensitive and 2) to study the impact of mechanical stretch on these cells in the presence and absence of hormones.
methodsFibroblasts obtained from biopsies of full thickness vagina of 3 women were cyclically biaxially stretched at a magnitude of 8 and 16% for 72 hours with or without 17-β-estradiol plus progesterone. Culture media was collected and total collagenase activity was measured in duplicate using a fluorogenic substrate degradation assay. Data were analyzed at the 0.05 level of significance using Student t-test.
resultsCells remained 90% viable throughout the experiments. Relative to the controls, hormonal treatment alone decreased collagenase activity (P=0.008). In the presence of mechanical stretch and in the absence of hormones, collagenase activity was increased (8% elongation, P=0.04; 16% elongation, P=0.001, respectively). The increase in collagenase activity was linearly correlated with magnitude (P<0.001). In the presence of hormones, the increase in enzyme activity by mechanical stretch was suppressed to baseline control levels (P=0.46). There was no difference in suppression by hormones by magnitude (P=0.48).
conclusionsVaginal connective tissue fibroblasts are mechanosensitive with increased collagenase activity in the presence of stretch. This degradative behavior is inhibited in the presence of hormones. The data provide a mechanism by which events that induce vaginal stretch may lead to progression of pelvic organ prolapse, particularly, in the absence of hormones. Further studies are needed to determine whether these events lead to tissue with inferior mechanical properties.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.