ArticleApplied and environmental microbiology2011
Lectin microarray reveals binding profiles of Lactobacillus casei strains in a comprehensive analysis of bacterial cell wall polysaccharides.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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26 citing papers in PubMed, 49 citations in OpenAlex.
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- Emerging technologies for single-cell glycomics.BBA advances · 2024Article
- Innate immune Galectin-7 specifically targets microbes that decorate themselves in blood group-like antigens.iScience · 2022Article
- Glycan profiling of the gut microbiota by Glycan-seq.ISME communications · 2022Article
- Glycan Profiling by Sequencing to Uncover Multicellular Communication: Launching Glycobiology in Single Cells and Microbiomes.Frontiers in cell and developmental biology · 2022Review
- Matrix glycoconjugate characterization in multispecies biofilms and bioaggregates from the environment by means of fluorescently-labeled lectins.Frontiers in microbiology · 2022Article
- Dectin-2 mediates phagocytosis of Lactobacillus paracasei KW3110 and IL-10 production by macrophages.Scientific reports · 2021Article
- Integrated analysis of glycan and RNA in single cells.iScience · 2021Article
- Application of the Combination of Soybean Lecithin and Whey Protein Concentrate 80 to Improve the Bile Salt and Acid Tolerance of Probiotics.Journal of microbiology and biotechnology · 2021Article
- Monoclonal Antibodies Generated against Glycoconjugates Recognize Chemical Linkers.Antibodies (Basel, Switzerland) · 2020Article
- Cell wall homeostasis in lactic acid bacteria: threats and defences.FEMS microbiology reviews · 2020Review
- Glycomics Microarrays Reveal Differential In Situ Presentation of the Biofilm Polysaccharide Poly-International journal of molecular sciences · 2020Article
- ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin.Glycoconjugate journal · 2020Article
- Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins.Frontiers in microbiology · 2019Review
- Mass spectrometric revival of an l-rhamnose- and d-galactose-specific lectin from a lost strain ofThe Journal of biological chemistry · 2018Article
- Surfaceome and Proteosurfaceome in Parietal Monoderm Bacteria: Focus on Protein Cell-Surface Display.Frontiers in microbiology · 2018Review
- Exposing the secrets of two well-known Lactobacillus casei phages, J-1 and PL-1, by genomic and structural analysis.Applied and environmental microbiology · 2014Article
- Chemical structure of the cell wall-associated polysaccharide of Bifidobacterium animalis subsp. lactis LKM512.Glycoconjugate journal · 2014Article
Corrections and comments
- Erratum issuedHirabarashi, Jun [corrected to Hirabayashi, Jun]
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously showed a pivotal role of the polysaccharide (PS) moiety in the cell wall of the Lactobacillus casei strain Shirota (YIT 9029) as a possible immune modulator (E. Yasuda M. Serata, and T. Sako, Appl. Environ. Microbiol. 74:4746-4755, 2008). To distinguish PS structures on the bacterial cell surface of individual strains in relation to their activities, it would be useful to have a rapid and high-throughput methodology. Recently, a new technique called lectin microarray was developed for rapid profiling of glycosylation in eukaryotic polymers and cell surfaces. Here, we report on the development of a simple and sensitive method based on this technology for direct analysis of intact bacterial cell surface glycomes. The method involves labeling bacterial cells with SYTOX Orange before incubation with the lectin microarray. After washing, bound cells are directly detected using an evanescent-field fluorescence scanner in a liquid phase. Using this method, we compared the cell surface glycomes from 16 different strains of L. casei. The patterns of lectin-binding affinity of most strains were found to be unique. There appears to be two types of lectin-binding profiles: the first is characterized by a few lectins, and the other is characterized by multiple lectins with different specificities. We also showed a dramatic change in the lectin-binding profile of a YIT 9029 derivative with a mutation in the cps1C gene, encoding a putative glycosyltransferase. In conclusion, the developed technique provided a novel strategy for rapid profiling and, more importantly, differentiating numerous bacterial strains with relevance to the biological functions of PS.
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