ArticleApplied and environmental microbiology2011
Disruption of a type II endonuclease (TDE0911) enables Treponema denticola ATCC 35405 to accept an unmethylated shuttle vector.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Expanded Functional Characterization and Optimization of Protein Expression in Treponema denticola Shuttle Plasmids.Molecular oral microbiology · 2026Article
- Emerging oralFrontiers in oral health · 2025Article
- Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.Molecular oral microbiology · 2023Article
- Characterization of Treponema denticola Major Surface Protein (Msp) by Deletion Analysis and Advanced Molecular Modeling.Journal of bacteriology · 2022Article
- Complete Genome Sequences of Three Human Oral Treponema parvum Isolates.Microbiology resource announcements · 2021Article
- Genetic Manipulations of Oral Spirochete Treponema denticola.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A pleiotropic role of FlaG in regulating the cell morphogenesis and flagellar homeostasis at the cell poles of Treponema denticola.Cellular microbiology · 2019Article
- The Role ofFrontiers in cellular and infection microbiology · 2019Article
- Spirochaete flagella hook proteins self-catalyse a lysinoalanine covalent crosslink for motility.Nature microbiology · 2016Article
- pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.Applied and environmental microbiology · 2016Article
- Premethylation of foreign DNA improves integrative transformation efficiency in Synechocystis sp. strain PCC 6803.Applied and environmental microbiology · 2015Article
- A Modified Shuttle Plasmid Facilitates Expression of a Flavin Mononucleotide-Based Fluorescent Protein in Treponema denticola ATCC 35405.Applied and environmental microbiology · 2015Article
- Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola.Applied and environmental microbiology · 2015Article
- Evidence that TP_0144 of Treponema pallidum is a thiamine-binding protein.Journal of bacteriology · 2015Article
- Inactivation of cyclic Di-GMP binding protein TDE0214 affects the motility, biofilm formation, and virulence of Treponema denticola.Journal of bacteriology · 2013Article
- Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.Applied and environmental microbiology · 2012Article
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2 authors at 1 institution in 1 country.
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Abstract
The oral spirochete Treponema denticola is associated with human periodontal disease. T. denticola ATCC 35405 and ATCC 33520 are two routinely used laboratory strains. Compared to T. denticola ATCC 33520, ATCC 35405 is more virulent but less accessible to genetic manipulations. For instance, the shuttle vectors of ATCC 33520 cannot be transformed into strain ATCC 35405. The lack of a shuttle vector has been a barrier to study the biology and virulence of T. denticola ATCC 35405. In this report, we hypothesize that T. denticola ATCC 35405 may have a unique DNA restriction-modification (R-M) system that prevents it from accepting the shuttle vectors of ATCC 33520 (e.g., the shuttle plasmid pBFC). To test this hypothesis, DNA restriction digestion, PCR, and Southern blot analyses were conducted to identify the differences between the R-M systems of these two strains. DNA restriction digestion analysis of these strains showed that only the cell extract from ATCC 35405 was able to digest pBFC. Consistently, PCR and Southern blot analyses revealed that the genome of T. denticola ATCC 35405 encodes three type II endonucleases that are absent in ATCC 33520. Among these three endonucleases, TDE0911 was predicted to cleave unmethylated double-stranded DNA and to be most likely responsible for the cleavage of unmethylated pBFC. In agreement with this prediction, the mutant of TDE0911 failed to cleave unmethylated pBFC plasmid, and it could accept the unmethylated shuttle vector. The study described here provides us with a new tool and strategy to genetically manipulate T. denticola, in particular ATCC 35405, and other strains that may carry similar endonucleases.
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