ArticleApplied and environmental microbiology2011
Validation of internal controls for extraction and amplification of nucleic acids from enteric viruses in water samples.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 100 citations in OpenAlex.
- High-Throughput Microfluidic Quantitative PCR Platform for the Simultaneous Quantification of Pathogens, Fecal Indicator Bacteria, and Microbial Source Tracking Markers.ACS ES&T water · 2023Article
- Surfactant Treatment for Efficient Gene Detection of Enteric Viruses and Indicators in Surface Water Concentrated by Ultrafiltration.Food and environmental virology · 2023Article
- Article
- Sewage surveillance for SARS-CoV-2: Molecular detection, quantification, and normalization factors.Current opinion in environmental science & health · 2022Review
- Selection of surrogate viruses for process control in detection of SARS-CoV-2 in wastewater.The Science of the total environment · 2022Article
- Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance.The Science of the total environment · 2022Review
- Comparative effectiveness of membrane technologies and disinfection methods for virus elimination in water: A review.The Science of the total environment · 2021Review
- Applicability of polyethylene glycol precipitation followed by acid guanidinium thiocyanate-phenol-chloroform extraction for the detection of SARS-CoV-2 RNA from municipal wastewater.The Science of the total environment · 2021Article
- Viruses in wastewater: occurrence, abundance and detection methods.The Science of the total environment · 2020Review
- Molecular diagnosis of COVID-19: Current situation and trend in China (Review).Experimental and therapeutic medicine · 2020Review
- Viral indicators for tracking domestic wastewater contamination in the aquatic environment.Water research · 2020Review
- Comprehensive Study on Enteric Viruses and Indicators in Surface Water in Kyoto, Japan, During 2014-2015 Season.Food and environmental virology · 2018Article
- Foodborne viruses: Detection, risk assessment, and control options in food processing.International journal of food microbiology · 2018Review
- Estimation of Human Norovirus Infectivity from Environmental Water Samples by In Situ Capture RT-qPCR Method.Food and environmental virology · 2018Article
- Oyster Contamination with Human Noroviruses Impacted by Urban Drainage and Seasonal Flooding in Vietnam.Food and environmental virology · 2018Article
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Concurrent Detection of Human Norovirus and Bacterial Pathogens in Water Samples from an Agricultural Region in Central California Coast.Frontiers in microbiology · 2017Article
- Article
- Aichi virus 1: environmental occurrence and behavior.Pathogens (Basel, Switzerland) · 2015Review
- Organic substances interfere with reverse transcription-quantitative PCR-based virus detection in water samples.Applied and environmental microbiology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inhibitors that reduce viral nucleic acid extraction efficiency and interfere with cDNA synthesis and/or polymerase activity affect the molecular detection of viruses in aquatic environments. To overcome these significant problems, we developed a methodology for assessing nucleic acid yields and DNA amplification efficiencies for environmental water samples. This involved adding particles of adenovirus type 5 and murine norovirus and newly developed primer-sharing controls, which are amplified with the same primer pairs and result in the same amplicon sizes as the targets, to these samples. We found that nucleic acid loss during the extraction process, rather than reverse transcription-PCR (RT-PCR) inhibition, more significantly attributed to underestimation of the presence of viral genomes in the environmental water samples tested in this study. Our success rate for satisfactorily amplifying viral RNAs and DNAs by RT-PCR was higher than that for obtaining adequate nucleic acid preparations. We found that inhibitory properties were greatest when we used larger sample volumes. A magnetic silica bead-based RNA extraction method effectively removed inhibitors that interfere with viral nucleic acid extraction and RT-PCR. To our knowledge, this is the first study to assess the inhibitory properties of environmental water samples by using both control virus particles and primer-sharing controls.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.