ArticleApplied and environmental microbiology2011
Secretion of anti-Plasmodium effector proteins from a natural Pantoea agglomerans isolate by using PelB and HlyA secretion signals.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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37 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.
- Evaluating paratransgenesis using engineered symbiotic bacteria for Plasmodium inhibition in mosquito vectors: A systematic review.PLoS neglected tropical diseases · 2026Pooled it
- Identifying Transgenic Approaches to Mitigate Vector Transmission of Malaria by Anopheles gambiae.Cureus · 2026Review
- Microbiota diversity ofmSphere · 2025Article
- A catalog of proteins released fromMicrobiology spectrum · 2025Article
- Harnessing engineered symbionts to combat concurrent malaria and arboviruses transmission.Nature communications · 2025Article
- The Role of Gut Microbiota in Shaping Immune Responses in Tephritidae Fruit Fly and Prospective Implications for Management.Neotropical entomology · 2025Review
- Diversity and Species Composition of Midgut Symbiotic Bacteria inJournal of tropical medicine · 2024Article
- The construction and evaluation of secretory expression engineering bacteria for the trans-Cry3Aa-T-HasA fusion protein against theFrontiers in cellular and infection microbiology · 2024Article
- Insect Microbial Symbionts: Ecology, Interactions, and Biological Significance.Microorganisms · 2023Review
- Bacterial Microbiota from Lab-Reared and Field-CapturedMicroorganisms · 2023Article
- Opposite Sides ofMicroorganisms · 2022Review
- Seasonal variation of microbiota composition in Anopheles gambiae and Anopheles coluzzii in two different eco-geographical localities in Cameroon.Medical and veterinary entomology · 2022Article
- Overview of paratransgenesis as a strategy to control pathogen transmission by insect vectors.Parasites & vectors · 2022Review
- Diversity of midgut bacteria in larvae and females of Aedes aegypti and Aedes albopictus from Gampaha District, Sri Lanka.Parasites & vectors · 2021Article
- Mosquito Trilogy: Microbiota, Immunity and Pathogens, and Their Implications for the Control of Disease Transmission.Frontiers in microbiology · 2021Review
- Impact of Gut Bacteria on the Infection and Transmission of Pathogenic Arboviruses by Biting Midges and Mosquitoes.Microbial ecology · 2020Article
- Identification and Characterization of pantocin wh-1, a Novel Cyclic Polypeptide Produced byMolecules (Basel, Switzerland) · 2020Article
- Molecular Characterization of Culturable Aerobic Bacteria in the Midgut of Field-CaughtBioMed research international · 2020Article
- CRISPR/Cas9-mediated gene deletion of the ompA gene in symbiotic Cedecea neteri impairs biofilm formation and reduces gut colonization of Aedes aegypti mosquitoes.PLoS neglected tropical diseases · 2019Article
- 16S Metagenomic Comparison ofThe American journal of tropical medicine and hygiene · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The insect-vectored disease malaria is a major world health problem. New control strategies are needed to supplement the current use of insecticides and medications. A genetic approach can be used to inhibit development of malaria parasites (Plasmodium spp.) in the mosquito host. We hypothesized that Pantoea agglomerans, a bacterial symbiont of Anopheles mosquitoes, could be engineered to express and secrete anti-Plasmodium effector proteins, a strategy termed paratransgenesis. To this end, plasmids that include the pelB or hlyA secretion signals from the genes of related species (pectate lyase from Erwinia carotovora and hemolysin A from Escherichia coli, respectively) were created and tested for their efficacy in secreting known anti-Plasmodium effector proteins (SM1, anti-Pbs21, and PLA2) in P. agglomerans and E. coli. P. agglomerans successfully secreted HlyA fusions of anti-Pbs21 and PLA2, and these strains are under evaluation for anti-Plasmodium activity in infected mosquitoes. Varied expression and/or secretion of the effector proteins was observed, suggesting that the individual characteristics of a particular effector may require empirical testing of several secretion signals. Importantly, those strains that secreted efficiently grew as well as wild-type strains under laboratory conditions and, thus, may be expected to be competitive with the native microbiota in the environment of the mosquito midgut.
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