ArticleJournal of bacteriology2011
Flagellar glycosylation in Burkholderia pseudomallei and Burkholderia thailandensis.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Phage resistance of Bordetella avium due to an altered cell wall results in increased susceptibility to the polypeptide antibiotics colistin and polymyxin B.Scientific reports · 2025Article
- Biochemical characterization of theFrontiers in microbiology · 2025Article
- Harnessing the acceptor substrate promiscuity of Clostridium botulinum Maf glycosyltransferase to glyco-engineer mini-flagellin protein chimeras.Communications biology · 2024Article
- Review
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- Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity.Microorganisms · 2020Review
- Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages.BMC microbiology · 2019Article
- Novel Role of VisP and the Wzz System during O-Antigen Assembly in Salmonella enterica Serovar Typhimurium Pathogenesis.Infection and immunity · 2018Article
- A ten gene-containing genomic island determines flagellin glycosylation: implication for its regulatory role in motility and virulence of Xanthomonas oryzae pv. oryzae.Molecular plant pathology · 2018Article
- Development of Rare Bacterial Monosaccharide Analogs for Metabolic Glycan Labeling in Pathogenic Bacteria.ACS chemical biology · 2016Article
- Flagellin glycosylation in Paenibacillus alvei CCM 2051T.Glycobiology · 2016Article
- Competition between Burkholderia pseudomallei and B. thailandensis.BMC microbiology · 2015Article
- The sweet tooth of bacteria: common themes in bacterial glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2014Review
- Identification of the flagellin glycosylation system in Burkholderia cenocepacia and the contribution of glycosylated flagellin to evasion of human innate immune responses.The Journal of biological chemistry · 2014Article
- Identification and characterization of glycoproteins on the spore surface of Clostridium difficile.Journal of bacteriology · 2014Article
- Gram-negative flagella glycosylation.International journal of molecular sciences · 2014Review
- Protection against experimental melioidosis following immunisation with a lipopolysaccharide-protein conjugate.Journal of immunology research · 2014Article
- Protection against experimental melioidosis following immunization with live Burkholderia thailandensis expressing a manno-heptose capsule.Clinical and vaccine immunology : CVI · 2013Article
- Posttranslational modification of flagellin FlaB in Shewanella oneidensis.Journal of bacteriology · 2013Article
- O-polysaccharide glycosylation is required for stability and function of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitans.Infection and immunity · 2012Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosylation of proteins is known to impart novel physical properties and biological roles to proteins from both eukaryotes and prokaryotes. In this study, gel-based glycoproteomics were used to identify glycoproteins of the potential biothreat agent Burkholderia pseudomallei and the closely related but nonpathogenic B. thailandensis. Top-down and bottom-up mass spectrometry (MS) analyses identified that the flagellin proteins of both species were posttranslationally modified by novel glycans. Analysis of proteins from two strains of each species demonstrated that B. pseudomallei flagellin proteins were modified with a glycan with a mass of 291 Da, while B. thailandensis flagellin protein was modified with related glycans with a mass of 300 or 342 Da. Structural characterization of the B. thailandensis carbohydrate moiety suggests that it is an acetylated hexuronic acid. In addition, we have identified through mutagenesis a gene from the lipopolysaccharide (LPS) O-antigen biosynthetic cluster which is involved in flagellar glycosylation, and inactivation of this gene eliminates flagellar glycosylation and motility in B. pseudomallei. This is the first report to conclusively demonstrate the presence of a carbohydrate covalently linked to a protein in B. pseudomallei and B. thailandensis, and it suggests new avenues to explore in order to examine the marked differences in virulence between these two species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.