ArticleThe Journal of biological chemistry2011
Clustering and activity tuning of Kv1 channels in myelinated hippocampal axons.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 49 citations in OpenAlex.
- Developmental transformations of Purkinje cells tracked by DNA electrokinetic mobility.Cell reports methods · 2025Article
- Neuromyotonia and CASPR2 Antibodies: Electrophysiological Clues to Disease Pathophysiology.Biomolecules · 2025Review
- Sleep Disturbances in Autoimmune Neurologic Diseases: Manifestation and Pathophysiology.Frontiers in neuroscience · 2021Review
- Comparison of KHandbook of experimental pharmacology · 2021Article
- Assembly and Function of the Juxtaparanodal Kv1 Complex in Health and Disease.Life (Basel, Switzerland) · 2020Review
- Copper chelation and autoimmunity differentially impact myelin in the hippocampal-prefrontal circuit.Journal of neuroimmunology · 2019Article
- Selective Axonal Expression of the Kv1 Channel Complex in Pre-myelinated GABAergic Hippocampal Neurons.Frontiers in cellular neuroscience · 2019Article
- Autoantibodies to Synaptic Receptors and Neuronal Cell Surface Proteins in Autoimmune Diseases of the Central Nervous System.Physiological reviews · 2017Review
- Suppression of Inflammatory Demyelinaton and Axon Degeneration through Inhibiting Kv3 Channels.Frontiers in molecular neuroscience · 2017Article
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- Caspr2 autoantibodies target multiple epitopes.Neurology(R) neuroimmunology & neuroinflammation · 2015Article
- Regulation of neurovascular coupling in autoimmunity to water and ion channels.Autoimmunity reviews · 2015Review
- Human embryonic stem cell-derived oligodendrocyte progenitors remyelinate the brain and rescue behavioral deficits following radiation.Cell stem cell · 2015Article
- Connecting the CNTNAP2 Networks with Neurodevelopmental Disorders.Molecular syndromology · 2015Review
- Clinical and experimental studies of potentially pathogenic brain-directed autoantibodies: current knowledge and future directions.Journal of neurology · 2015Review
- Physiological and pathological functions of mechanosensitive ion channels.Molecular neurobiology · 2014Review
- The node of Ranvier in CNS pathology.Acta neuropathologica · 2014Review
- Structural organization and function of mouse photoreceptor ribbon synapses involve the immunoglobulin protein synaptic cell adhesion molecule 1.The Journal of comparative neurology · 2014Article
- Ankyrin-G directly binds to kinesin-1 to transport voltage-gated Na+ channels into axons.Developmental cell · 2014Article
- Neuronal expression of GalNAc transferase is sufficient to prevent the age-related neurodegenerative phenotype of complex ganglioside-deficient mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Precise localization of axonal ion channels is crucial for proper electrical and chemical functions of axons. In myelinated axons, Kv1 (Shaker) voltage-gated potassium (Kv) channels are clustered in the juxtaparanodal regions flanking the node of Ranvier. The clustering can be disrupted by deletion of various proteins in mice, including contactin-associated protein-like 2 (Caspr2) and transient axonal glycoprotein-1 (TAG-1), a glycosylphosphatidylinositol-anchored cell adhesion molecule. However, the mechanism and function of Kv1 juxtaparanodal clustering remain unclear. Here, using a new myelin coculture of hippocampal neurons and oligodendrocytes, we report that tyrosine phosphorylation plays a critical role in TAG-1-mediated clustering of axonal Kv1.2 channels. In the coculture, myelin specifically ensheathed axons but not dendrites of hippocampal neurons and clustered endogenous axonal Kv1.2 into internodes. The trans-homophilic interaction of TAG-1 was sufficient to position Kv1.2 clusters on axonal membranes in a neuron/HEK293 coculture. Mutating a tyrosine residue (Tyr⁴⁵⁸) in the Kv1.2 C terminus or blocking tyrosine phosphorylation disrupted myelin- and TAG-1-mediated clustering of axonal Kv1.2. Furthermore, Kv1.2 voltage dependence and activation threshold were reduced by TAG-1 coexpression. This effect was eliminated by the Tyr⁴⁵⁸ mutation or by cholesterol depletion. Taken together, our studies suggest that myelin regulates both trafficking and activity of Kv1 channels along hippocampal axons through TAG-1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.