ArticleJournal of neuroscience methods2011
Chondroitinase activity can be transduced by a lentiviral vector in vitro and in vivo.
Article in Journal of neuroscience methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Beyond Transplantation: Engineering Neural Cell Therapies and Combination Strategies for Spinal Cord Repair.Brain sciences · 2026Review
- Agrin Inhibition in Enteric Neural Stem Cells Enhances Their Migration Following Colonic Transplantation.Stem cells translational medicine · 2024Article
- Modified adeno-associated virus targets the bacterial enzyme chondroitinase ABC to select mouse neuronal populations in vivo using the Cre-LoxP system.The European journal of neuroscience · 2021Article
- Substrate Specificity and Biochemical Characteristics of an Engineered Mammalian Chondroitinase ABC.ACS omega · 2021Article
- Article
- Secretion of a mammalian chondroitinase ABC aids glial integration at PNS/CNS boundaries.Scientific reports · 2020Article
- Prospects of siRNA cocktails as tools for modifying multiple gene targets in the injured spinal cord.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- Partial Reconstruction of the Nigrostriatal Circuit along a Preformed Molecular Guidance Pathway.Molecular therapy. Methods & clinical development · 2019Article
- On the development of optical peripheral nerve interfaces.Neural regeneration research · 2019Review
- The Biology of Regeneration Failure and Success After Spinal Cord Injury.Physiological reviews · 2018Review
- Postinjury Induction of Activated ErbB2 Selectively Hyperactivates Denervated Schwann Cells and Promotes Robust Dorsal Root Axon Regeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Combined chondroitinase and KLF7 expression reduce net retraction of sensory and CST axons from sites of spinal injury.Neurobiology of disease · 2017Article
- Transplantation of neural progenitor cells in chronic spinal cord injury.Neuroscience · 2016Article
- Guiding migration of transplanted glial progenitor cells in the injured spinal cord.Scientific reports · 2016Article
- Biomaterial Approaches to Enhancing Neurorestoration after Spinal Cord Injury: Strategies for Overcoming Inherent Biological Obstacles.BioMed research international · 2015Review
- Large-scale chondroitin sulfate proteoglycan digestion with chondroitinase gene therapy leads to reduced pathology and modulates macrophage phenotype following spinal cord contusion injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Chondroitin sulfate proteoglycans in the nervous system: inhibitors to repair.BioMed research international · 2014Review
- Chondroitin sulfate proteoglycans potently inhibit invasion and serve as a central organizer of the brain tumor microenvironment.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The bacterial enzyme chondroitinase ABC (ChABC), which cleaves chondroitin sulfate glycosaminoglycan chains, can degrade inhibitory scar tissue formed following spinal cord injury, thereby promoting axonal growth and regeneration. However, delivering the active enzyme for prolonged periods presents practical limitations. To overcome these problems, we prepared a lentiviral vector (LV) encoding chondroitinase AC (Chase) together with the green fluorescent protein (GFP) reporter (Chase/LV) and demonstrated its expression and enzymatic activity in vitro and in vivo. Neural precursor cells infected with Chase/LV expressed the GFP reporter at levels that increased dramatically with time in culture. Enzymatic activity from the supernatant of the infected cells was demonstrated by dot blot assay using an antibody that recognizes the digested form of CSPG and was compared with the bacterial ChABC enzyme. Chick DRG cultures plated adjacent to the CSPG border and incubated with supernatant from Chase/LV-infected cells showed neurites growing into the CSPG area, a response similar to that after treatment with ChABC. In contrast, in control cultures, the neurites turned to avoid the inhibitory CSPG interface. Degradation of CSPG in these cultures was confirmed by specific CSPG antibodies. A single injection of Chase/LV into the spinal cord resulted in sustained secretion of the enzyme, whose activity was detected for 8 weeks by expression of GFP and evidence of the digested form of CSPG. This study demonstrates the efficacy of the Chase/LV vector and its potential as a therapeutic tool to reduce scar inhibition and promote axonal growth and repair following central nervous system injury.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.