ArticleProtein expression and purification2011
Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography.
Article in Protein expression and purification, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- Influence of HIV-1 Genomic RNA on the Formation of Gag Biomolecular Condensates.Journal of molecular biology · 2023Article
- Essential role of Wtip in mouse development and maintenance of the glomerular filtration barrier.American journal of physiology. Renal physiology · 2022Article
- Zinc is a master-regulator of sperm function associated with binding, motility, and metabolic modulation during porcine sperm capacitation.Communications biology · 2022Article
- Development of a Bio-Layer Interferometry-Based Protease Assay Using HIV-1 Protease as a Model.Viruses · 2021Article
- Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies.International journal of molecular sciences · 2021Article
- Isoquinoline thiosemicarbazone displays potent anticancer activity withRSC medicinal chemistry · 2020Article
- AMolecular and cellular biology · 2020Article
- Conserved cysteines in Mason-Pfizer monkey virus capsid protein are essential for infectious mature particle formation.Virology · 2018Article
- Article
- A Conserved Metal Binding Motif in the Bacillus subtilis Competence Protein ComFA Enhances Transformation.Journal of bacteriology · 2017Article
- Nucleic Acid Binding by Mason-Pfizer Monkey Virus CA Promotes Virus Assembly and Genome Packaging.Journal of virology · 2016Article
- Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli.Current protocols in protein science · 2012Article
- Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli.Current protocols in protein science · 2004Article
- Comparative interactomes of HSF1 in stress and disease reveal a role for CTCF in HSF1-mediated gene regulation.The Journal of biological chemistryArticle
- Challenges and opportunities in the purification of recombinant tagged proteins.Biotechnology advancesReview
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Authors and funding
5 authors.
Funding
Abstract
Heterologous proteins are frequently purified by immobilized metal ion affinity chromatography (IMAC) based on their modification with a hexa-histidine affinity tag (His-tag). The terminal His-tag can, however, alter functional properties of the tagged protein. Numerous strategies for the tag removal have been developed including chemical treatment and insertion of protease target sequences in the protein sequence. Instead of using these approaches, we took an advantage of natural interaction of zinc finger domains with metal ions to purify functionally similar retroviral proteins from two different retroviruses. We found that these proteins exhibited significantly different affinities to the immobilized metal ions, despite that both contain the same type of zinc finger motif (i.e., CCHC). While zinc finger proteins may differ in biochemical properties, the multitude of IMAC platforms should allow relatively simple yet specific method for their isolation in native state.
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