ArticleBMC genomics2011
Identification of factors required for meristem function in Arabidopsis using a novel next generation sequencing fast forward genetics approach.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Review
- Article
- Virulence and pathogenicity determinants in whole genome sequence ofFrontiers in microbiology · 2023Article
- Candidate powdery mildew resistance gene in wheat landrace cultivar Hongyoumai discovered using SLAF and BSR-seq.BMC plant biology · 2022Article
- Article
- Arabidopsis mTERF9 protein promotes chloroplast ribosomal assembly and translation by establishing ribonucleoprotein interactions in vivo.Nucleic acids research · 2021Article
- Review
- Clubroot resistance gene Rcr6 in Brassica nigra resides in a genomic region homologous to chromosome A08 in B. rapa.BMC plant biology · 2019Article
- Analysis of genome-wide variants through bulked segregant RNA sequencing reveals a major gene for resistance to Plasmodiophora brassicae in Brassica oleracea.Scientific reports · 2018Article
- A nuclear-encoded protein, mTERF6, mediates transcription termination of rpoA polycistron for plastid-encoded RNA polymerase-dependent chloroplast gene expression and chloroplast development.Scientific reports · 2018Article
- Control of organelle gene expression by the mitochondrial transcription termination factor mTERF22 in Arabidopsis thaliana plants.PloS one · 2018Article
- Forward Genetics by Sequencing EMS Variation-Induced Inbred Lines.G3 (Bethesda, Md.) · 2017Article
- Functional relationship between mTERF4 and GUN1 in retrograde signaling.Journal of experimental botany · 2016Article
- Next-generation sequencing for identification of candidate genes for Fusarium wilt and sterility mosaic disease in pigeonpea (Cajanus cajan).Plant biotechnology journal · 2016Article
- Mapping QTLs for Salt Tolerance in Rice (Oryza sativa L.) by Bulked Segregant Analysis of Recombinant Inbred Lines Using 50K SNP Chip.PloS one · 2016Article
- Mining candidate genes associated with powdery mildew resistance in cucumber via super-BSA by specific length amplified fragment (SLAF) sequencing.BMC genomics · 2015Article
- Variant mapping and mutation discovery in inbred mice using next-generation sequencing.BMC genomics · 2015Article
- Scanning the effects of ethyl methanesulfonate on the whole genome of Lotus japonicus using second-generation sequencing analysis.G3 (Bethesda, Md.) · 2015Article
- Using next-generation sequencing to isolate mutant genes from forward genetic screens.Nature reviews. Genetics · 2014Review
- Genomic variation in Arabidopsis: tools and insights from next-generation sequencing.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2014Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhenotype-driven forward genetic experiments are powerful approaches for linking phenotypes to genomic elements but they still involve a laborious positional cloning process. Although sequencing of complete genomes now becomes available, discriminating causal mutations from the enormous amounts of background variation remains a major challenge.
methodTo improve this, we developed a universal two-step approach, named 'fast forward genetics', which combines traditional bulk segregant techniques with targeted genomic enrichment and next-generation sequencing technology
resultsAs a proof of principle we successfully applied this approach to two Arabidopsis mutants and identified a novel factor required for stem cell activity.
conclusionWe demonstrated that the 'fast forward genetics' procedure efficiently identifies a small number of testable candidate mutations. As the approach is independent of genome size, it can be applied to any model system of interest. Furthermore, we show that experiments can be multiplexed and easily scaled for the identification of multiple individual mutants in a single sequencing run.
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