ArticleVirology journal2011
HPV16 E2 could act as down-regulator in cellular genes implicated in apoptosis, proliferation and cell differentiation.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Integrative Bioinformatic Approach for microRNA Interactome Networks in Human Papillomavirus-16 Infection.The Eurasian journal of medicine · 2025Article
- Alteration of the IFN-Pathway by Human Papillomavirus Proteins: Antiviral Immune Response Evasion Mechanism.Biomedicines · 2022Review
- The SMC5/6 Complex Represses the Replicative Program of High-Risk Human Papillomavirus Type 31.Pathogens (Basel, Switzerland) · 2020Article
- The association of integration patterns of human papilloma virus and single nucleotide polymorphisms on immune- or DNA repair-related genes in cervical cancer patients.Scientific reports · 2019Article
- Article
- Multiple Routes to Oncogenesis Are Promoted by the Human Papillomavirus-Host Protein Network.Cancer discovery · 2018Article
- Prevalence of the integration status for human papillomavirus 16 in esophageal carcinoma samples.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2018Article
- HPV16-E2 protein modifies self-renewal and differentiation rate in progenitor cells of human immortalized keratinocytes.Virology journal · 2017Article
- Involvement of Brd4 in different steps of the papillomavirus life cycle.Virus research · 2017Review
- PDGF beta targeting in cervical cancer cells suggest a fine-tuning of compensatory signalling pathways to sustain tumourigenic stimulation.Journal of cellular and molecular medicine · 2015Article
- Regulation of human genome expression and RNA splicing by human papillomavirus 16 E2 protein.Virology · 2014Article
- Regulation of HPV16 E6 and MCL1 by SF3B1 inhibitor in head and neck cancer cells.Scientific reports · 2014Article
- E2 proteins of high risk human papillomaviruses down-modulate STING and IFN-κ transcription in keratinocytes.PloS one · 2014Article
- The papillomavirus E2 proteins.Virology · 2013Review
- HPV related diseases in males: a heavy vaccine-preventable burden.Journal of preventive medicine and hygiene · 2013Review
- HPV-Based Screening, Triage, Treatment, and Followup Strategies in the Management of Cervical Intraepithelial Neoplasia.Obstetrics and gynecology international · 2013Article
- Expression of mitochondrial non-coding RNAs (ncRNAs) is modulated by high risk human papillomavirus (HPV) oncogenes.The Journal of biological chemistry · 2012Article
- The HPV E2-Host Protein-Protein Interactions: A Complex Hijacking of the Cellular Network.The open virology journal · 2012Article
- Co-expression of metalloproteinases 11 and 12 in cervical scrapes cells from cervical precursor lesions.International journal of clinical and experimental pathology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman Papillomavirus (HPV) E2 plays several important roles in the viral cycle, including the transcriptional regulation of the oncogenes E6 and E7, the regulation of the viral genome replication by its association with E1 helicase and participates in the viral genome segregation during mitosis by its association with the cellular protein Brd4. It has been shown that E2 protein can regulate negative or positively the activity of several cellular promoters, although the precise mechanism of this regulation is uncertain. In this work we constructed a recombinant adenoviral vector to overexpress HPV16 E2 and evaluated the global pattern of biological processes regulated by E2 using microarrays expression analysis.
resultsThe gene expression profile was strongly modified in cells expressing HPV16 E2, finding 1048 down-regulated genes, and 581 up-regulated. The main cellular pathway modified was WNT since we found 28 genes down-regulated and 15 up-regulated. Interestingly, this pathway is a convergence point for regulating the expression of genes involved in several cellular processes, including apoptosis, proliferation and cell differentiation; MYCN, JAG1 and MAPK13 genes were selected to validate by RT-qPCR the microarray data as these genes in an altered level of expression, modify very important cellular processes. Additionally, we found that a large number of genes from pathways such as PDGF, angiogenesis and cytokines and chemokines mediated inflammation, were also modified in their expression.
conclusionsOur results demonstrate that HPV16 E2 has regulatory effects on cellular gene expression in HPV negative cells, independent of the other HPV proteins, and the gene profile observed indicates that these effects could be mediated by interactions with cellular proteins. The cellular processes affected suggest that E2 expression leads to the cells in to a convenient environment for a replicative cycle of the virus.
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