ArticleBiochemical Society transactions2011
Crystallizing membrane proteins for structure-function studies using lipidic mesophases.
Article in Biochemical Society transactions, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Thromboxane AMethods in molecular biology (Clifton, N.J.) · 2022Article
- G protein-coupled receptors: structure- and function-based drug discovery.Signal transduction and targeted therapy · 2021Review
- Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies.PloS one · 2021Article
- Structural basis for the coordination of cell division with the synthesis of the bacterial cell envelope.Protein science : a publication of the Protein Society · 2019Review
- Structural Studies of G Protein-Coupled Receptors.Molecules and cells · 2015Review
- A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.Acta crystallographica. Section F, Structural biology communications · 2015Review
- Amphipols for each season.The Journal of membrane biology · 2014Review
- High-resolution structure of a membrane protein transferred from amphipol to a lipidic mesophase.The Journal of membrane biology · 2014Article
- Surfactant bilayers maintain transmembrane protein activity.Biophysical journal · 2014Article
- Liposome display for in vitro selection and evolution of membrane proteins.Nature protocols · 2014Article
- An X-ray transparent microfluidic platform for screening of the phase behavior of lipidic mesophases.The Analyst · 2013Article
- An overview of biological macromolecule crystallization.International journal of molecular sciences · 2013Review
- Towards protein-crystal centering using second-harmonic generation (SHG) microscopy.Acta crystallographica. Section D, Biological crystallography · 2013Article
- Micro-crystallography comes of age.Current opinion in structural biology · 2012Review
- Use of a robot for high-throughput crystallization of membrane proteins in lipidic mesophases.Journal of visualized experiments : JoVE · 2012Article
- Membrane protein structure determination using crystallography and lipidic mesophases: recent advances and successes.Biochemistry · 2012Article
- Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis.Structure (London, England : 1993) · 2012Article
- Physiology and emerging biochemistry of the glucagon-like peptide-1 receptor.Experimental diabetes research · 2012Review
- Toward structure determination using membrane-protein nanocrystals and microcrystals.Methods (San Diego, Calif.) · 2011Review
- Crystallization chaperone strategies for membrane proteins.Methods (San Diego, Calif.) · 2011Review
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Abstract
The lipidic cubic phase method for crystallizing membrane proteins has posted some high-profile successes recently. This is especially true in the area of G-protein-coupled receptors, with six new crystallographic structures emerging in the last 3½ years. Slowly, it is becoming an accepted method with a proven record and convincing generality. However, it is not a method that is used in every membrane structural biology laboratory and that is unfortunate. The reluctance in adopting it is attributable, in part, to the anticipated difficulties associated with handling the sticky viscous cubic mesophase in which crystals grow. Harvesting and collecting diffraction data with the mesophase-grown crystals is also viewed with some trepidation. It is acknowledged that there are challenges associated with the method. However, over the years, we have worked to make the method user-friendly. To this end, tools for handling the mesophase in the pico- to nano-litre volume range have been developed for efficient crystallization screening in manual and robotic modes. Glass crystallization plates have been built that provide unparalleled optical quality and sensitivity to nascent crystals. Lipid and precipitant screens have been implemented for a more rational approach to crystallogenesis, such that the method can now be applied to a wide variety of membrane protein types and sizes. In the present article, these assorted advances are outlined, along with a summary of the membrane proteins that have yielded to the method. The challenges that must be overcome to develop the method further are described.
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