ArticleVirus genes2011
Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein.
Article in Virus genes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 35 citations in OpenAlex.
- Co-Circulation of Divergent Strains Supports Vector-Mediated Transmission of Rodent Hepacivirus J (Viruses · 2026Article
- Maternal antibody-mediated elimination of a Puumala hantavirus outbreak in a bank vole colony.PLoS pathogens · 2026Article
- Tropism ofViruses · 2023Article
- Characterization of a Panel of Cross-Reactive Hantavirus Nucleocapsid Protein-Specific Monoclonal Antibodies.Viruses · 2023Article
- Cluster of human Puumala orthohantavirus infections due to indoor exposure?-An interdisciplinary outbreak investigation.Zoonoses and public health · 2022Article
- The Bank Vole (Viruses · 2021Article
- Genetic Diversity ofViruses · 2021Article
- Phylogeographic diversity and hybrid zone of Hantaan orthohantavirus collected in Gangwon Province, Republic of Korea.PLoS neglected tropical diseases · 2020Article
- Isolation and characterization of new Puumala orthohantavirus strains from Germany.Virus genes · 2020Article
- Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment.Pathogens (Basel, Switzerland) · 2020Article
- Field vole-associated Traemmersee hantavirus from Germany represents a novel hantavirus species.Virus genes · 2019Article
- Secondary contact between diverged host lineages entails ecological speciation in a European hantavirus.PLoS biology · 2019Article
- Phylogenetic analysis of Puumala virus strains from Central Europe highlights the need for a full-genome perspective on hantavirus evolution.Virus genes · 2017Article
- Revised time scales of RNA virus evolution based on spatial information.Proceedings. Biological sciences · 2017Article
- Puumala hantavirus infections in bank vole populations: host and virus dynamics in Central Europe.BMC ecology · 2017Article
- Validation of the Puumala virus rapid field test for bank voles in Germany.Epidemiology and infection · 2017Article
- Host-Associated Absence of Human Puumala Virus Infections in Northern and Eastern Germany.Emerging infectious diseases · 2017Article
- Spatiotemporal dynamics of Puumala hantavirus associated with its rodent host, Myodes glareolus.Evolutionary applications · 2015Article
- Complete genome of a Puumala virus strain from Central Europe.Virus genes · 2015Article
- Identification of factors influencing the Puumala virus seroprevalence within its reservoir in aMontane Forest Environment.Viruses · 2014Article
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Authors and funding
15 authors at 11 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Puumala virus (PUUV) is the predominant hantavirus species in Germany causing large numbers of mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS). During an outbreak in South-East Germany in 2004 a novel PUUV subtype designated Bavaria was identified as the causative agent of HFRS in humans [1]. Here we present a molecular characterization of this PUUV strain by investigating novel partial and almost entire nucleocapsid (N) protein-encoding small (S-) segment sequences and partial medium (M-) segment sequences from bank voles (Myodes glareolus) trapped in Lower Bavaria during 2004 and 2005. Phylogenetic analyses confirmed their classification as subtype Bavaria, which is further subdivided into four geographical clusters. The entire N protein, harbouring an amino-terminal hexahistidine tag, of the Bavarian strain was produced in yeast Saccharomyces cerevisiae and showed a slightly different reactivity with N-specific monoclonal antibodies, compared to the yeast-expressed N protein of the PUUV strain Vranica/Hällnäs. Endpoint titration of human sera from different parts of Germany and from Finland revealed only very slight differences in the diagnostic value of the different recombinant proteins. Based on the novel N antigen indirect and monoclonal antibody capture IgG-ELISAs were established. By using serum panels from Germany and Finland their validation demonstrated a high sensitivity and specificity. In summary, our investigations demonstrated the Bavarian PUUV strain to be genetically divergent from other PUUV strains and the potential of its N protein for diagnostic applications.
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