ArticleInvestigative ophthalmology & visual science2011
Initial in vitro investigation of the human immune response to corneal cells from genetically engineered pigs.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 51 citations in OpenAlex.
- Cell and tissue xenotransplantation: clinical purpose determines translational strategy and therapeutic endpoints.Clinical transplantation and research · 2026Review
- GM-CSF and IL-1α secreted by cryopreserved porcine skin promote angiogenesis in burn wounds by activating the JAK2/STAT3 pathway.American journal of translational research · 2026Article
- Human herpesvirus 7 epithelial keratitis with geographic-dendritic ulceration.BMC infectious diseases · 2025Article
- Immunology in corneal transplantation-From homeostasis to graft rejection.Transplantation reviews (Orlando, Fla.) · 2025Review
- Genetically modified pigs with α1,3-galactosyltransferase knockout and beyond: a comprehensive review of xenotransplantation strategies.Frontiers in immunology · 2025Review
- Animal Models in Eye Research: Focus on Corneal Pathologies.International journal of molecular sciences · 2023Review
- Human immune reactivity of GGTA1/CMAH/A3GALT2 triple knockout Yucatan miniature pigs.Transgenic research · 2021Article
- Corneal xenotransplantation: Where are we standing?Progress in retinal and eye research · 2021Review
- Justification of specific genetic modifications in pigs for clinical organ xenotransplantation.Xenotransplantation · 2019Review
- Predictive biomarkers for graft rejection in pig-to-non-human primate corneal xenotransplantation.Xenotransplantation · 2019Article
- Reducing porcine corneal graft rejection, with an emphasis on porcine endogenous retrovirus transmission safety: a review.International journal of ophthalmology · 2019Review
- B cell phenotypes in baboons with pig artery patch grafts receiving conventional immunosuppressive therapy.Transplant immunology · 2018Article
- Antigenicity of tissues and organs from GGTA1/CMAH/β4GalNT2 triple gene knockout pigs.Journal of biomedical research · 2018Article
- Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation.Frontiers in immunology · 2018Review
- Transplantation of hepatocytes from genetically engineered pigs into baboons.Xenotransplantation · 2017Article
- Development of retrocorneal membrane following pig-to-monkey penetrating keratoplasty.Xenotransplantation · 2017Article
- Effect of Rho-kinase Inhibitor, Y27632, on Porcine Corneal Endothelial Cell Culture, Inflammation and Immune Regulation.Ocular immunology and inflammation · 2016Article
- Distinctive Leukocyte Subpopulations According to Organ Type in Cynomolgus Macaques.Comparative medicine · 2016Article
- Expression of NeuGc on Pig Corneas and Its Potential Significance in Pig Corneal Xenotransplantation.Cornea · 2016Article
- In vitro testing of an anti-CD40 monoclonal antibody, clone 2C10, in primates and pigs.Transplant immunology · 2015Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo compare the in vitro human humoral and cellular immune responses to wild-type (WT) pig corneal endothelial cells (pCECs) with those to pig aortic endothelial cells (pAECs). These responses were further compared with CECs from genetically engineered pigs (α1,3-galactosyltransferase gene-knockout [GTKO] pigs and pigs expressing a human complement-regulatory protein [CD46]) and human donors.
methodsThe expression of Galα1,3Gal (Gal), swine leukocyte antigen (SLA) class I and class II on pCECs and pAECs, with or without activation by porcine IFN-γ, was tested by flow cytometry. Pooled human serum was used to measure IgM/IgG binding to and complement-dependent cytotoxicity (CDC) to cells from WT, GTKO, and GTKO/CD46 pigs. The human CD4(+) T-cell response to cells from WT, GTKO, GTKO/CD46 pigs and human was tested by mixed lymphocyte reaction (MLR).
resultsThere was a lower level of expression of the Gal antigen and of SLA class I and II on the WT pCECs than on the WT pAECs, resulting in less antibody binding and reduced human CD4(+) T-cell proliferation. However, lysis of the WT pCECs was equivalent to that of the pAECs, suggesting more susceptibility to injury. There were significantly weaker humoral and cellular responses to the pCECs from GTKO/CD46 pigs compared with the WT pCECs, although the cellular response to the GTKO/CD46 pCECs was greater than to the human CECs.
conclusionsThese data provide the first report of in vitro investigations of CECs from genetically engineered pigs and suggest that pig corneas may provide an acceptable alternative to human corneas for clinical transplantation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.